Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

  • 中文名称：
    牛单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒
  • 货号：
    CSB-E16221B
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Bovine CCL2 ELISA Kit is a tool for the quantification of C-C motif chemokine 2 (CCL2) in bovine serum, plasma, and tissue homogenates. CCL2 is a protein that plays a critical role in immune responses, particularly in the recruitment of immune cells to sites of inflammation. This ELISA kit detects CCL2 with high sensitivity, with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 0.039 ng/mL.

    The assay principle is a sandwich measurement, providing quantitative results that can be obtained in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a cost-effective and efficient solution for researchers.

    CCL2 has been shown to be a reliable biomarker of immune responses. and this kit provides researchers with a precise and reliable method to accurately measure CCL2 levels in bovine samples.

  • 别名：
    CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Acidic seminal fluid protein ELISA Kit; Monocyte chemotactic protein 1A ELISA Kit; MCP-1 ELISA Kit; MCP-1A ELISA Kit; Small-inducible cytokine A2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine RBP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 82-95
    1:2 Average % 98
    Range % 94-102
    1:4 Average % 97
    Range % 90-105
    1:8 Average % 86
    Range % 82-92
  • 回收率：
    The recovery of bovine RBP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 88-96
    EDTA plasma (n=4) 100 96-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 1.921 1.890 1.906 1.792
    5 1.703 1.749 1.726 1.612
    2.5 1.345 1.431 1.388 1.274
    1.25 1.023 1.075 1.049 0.935
    0.625 0.671 0.722 0.697 0.583
    0.312 0.429 0.440 0.435 0.321
    0.156 0.282 0.289 0.286 0.172
    0 0.111 0.116 0.114 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
  • 基因功能参考文献：
    1. CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy. PMID: 28816360
    2. resence of type 2 diabetes negatively affects the work of cytokines and markers of fibrosis, as evidenced by higher levels of interleukin-1beta and monocyte chemoattractant protein-1, compared with patients without diabetes PMID: 25020170
    3. Studied SNPs in the bovine toll-like receptor 4 (TLR4) and monocyte chemo attractant protein-1(CCL2) genes. PMID: 24813219
    4. The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID: 23184004
    5. immune cells and endothelial cells can interact cooperatively to increase CCL2 secretion in the corpus luteum PMID: 15659710

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    KEGG: bta:281043

    STRING: 9913.ENSBTAP00000013146

    UniGene: Bt.2408