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  4. Bovine neuropeptide Y (NPY) ELISA kit

Bovine neuropeptide Y (NPY) ELISA kit

  • 中文名称：
    牛神经肽Y(NPY)酶联免疫试剂盒
  • 货号：
    CSB-E08171b
  • 规格：
    96T/48T
  • 价格：
    ￥4800/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine NPY ELISA Kit was designed for the quantitative measurement of Bovine NPY protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    NPY ELISA Kit; Pro-neuropeptide Y [Cleaved into: Neuropeptide Y ELISA Kit; Neuropeptide tyrosine ELISA Kit; NPY); C-flanking peptide of NPY ELISA Kit; CPON)] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %87-93
    1:2Average %102
    Range %98-105
    1:4Average %90
    Range %86-96
    1:8Average %91
    Range %88-97
  • 回收率：
    The recovery of bovine NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-94
    EDTA plasma (n=4)9187-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.604 2.490 2.547 2.444
    102.113 2.221 2.167 2.064
    51.657 1.711 1.684 1.581
    2.51.114 1.056 1.085 0.982
    1.250.712 0.687 0.700 0.597
    0.6250.413 0.428 0.421 0.318
    0.3120.278 0.260 0.269 0.166
    00.105 0.101 0.103
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
  • 基因功能参考文献：
    1. Hormonal gene expression involved in residual feed intake in dairy cows may be related to the molecular regulation of the leptin-NPY and insulin signaling pathways. PMID: 26231801
    2. Data indicate that increased body weight gain during juvenile development accelerates sexual maturation in heifers, coincident with reciprocal changes in circulating concentrations of leptin and hypothalamic neuropeptide Y (NPY) release. PMID: 25326602
    3. There is a strong association between putative favorable allelic variants (SNP) of neuropeptide Y, leptin, and IGF-1 genes, and residual feed intake, when animals were grazing on a high-quality, high-availability pasture. PMID: 23881687
    4. The NPY SNP (NPY1) was associated with the prevalence of the animal being in calf 100 d after calving and 305-d milk yield in the first lactation. PMID: 21700051
    5. NPY tended to increase serum growth hormone, which appeared to be a consequence of increased pulse amplitude. Infusion of NPY also increased CSF growth hormone-releasing hormone. PMID: 19632078
    6. The effect of single nucleotide polymorphisms in 6 genes and their associations with production factors in beef cattle are reported. PMID: 17785604
    7. Data show that PKA and PKC pathways are involved in the differential regulation of production of the neuropeptides (Met)enkephalin, galanin, somatostatin, NPY, and VIP. PMID: 18619673

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  • 亚细胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
  • 蛋白家族：
    NPY family
  • 数据库链接：