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  4. Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit

Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit

  • 中文名称：
    牛α1酸性糖蛋白(α1-AGP)酶联免疫试剂盒
  • 货号：
    CSB-E08579b
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine ORM1 ELISA Kit was designed for the quantitative measurement of Bovine ORM1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.

  • 别名：
    ORM1 ELISA kit; AGPAlpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
  • 缩写：
    ORM1
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 ng/mL-4000 ng/mL
  • 灵敏度：
    15.6 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 102  
    Range % 94-106  
    1:2000 Average % 92  
    Range % 84-97  
    1:4000 Average % 101  
    Range % 95-104  
    1:8000 Average % 97  
    Range % 92-101  
  • 回收率：
    The recovery of bovine α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 88-99  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    4000 0.229 0.225 0.227    
    2000 0.450 0.459 0.455    
    1000 0.698 0.656 0.677    
    500 1.016 0.987 1.002    
    250 1.264 1.243 1.254    
    125 1.572 1.514 1.543    
    62.5 1.671 1.634 1.653    
    0 1.815 1.789 1.802    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 基因功能参考文献：
    1. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
    2. results provide evidence that locally produced alpha-1 acid glycoprotein may be involved in protecting sperm from phagocytosis by polymorphonuclear neutrophils in the bovine oviduct PMID: 24931131
    3. Studied the regulation and immune function of AGP in cultured bovine oviduct epithelial cells. PMID: 25123565
    4. As in human AGP and several other lipocalins, a temperature-induced molten globule state has been observed in the denaturation pathway of bovine AGP. PMID: 24530968
    5. In the mitochondrial membrane, both AGP and MLQ proteins are almost quantitatively bound to ATP synthase. PMID: 17575325
    6. Alpha1-acid glycoprotein isoforms that can be detected in bovine milk are reported. PMID: 17692138
    7. study provides evidence that the expression of CD18 was downregulated by the plasma glycoforms of alpha-1 acid glycoprotein and inhibited the chemotaxis of monocytes PMID: 18577362

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 数据库链接：