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  4. Canine Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Canine Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

  • 中文名称：
    犬心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒 Kit
  • 货号：
    CSB-E08772c
  • 规格：
    96T/48T
  • 价格：
    ￥4800/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog cTn-I ELISA Kit was designed for the quantitative measurement of Dog cTn-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
  • 缩写：
    cTn-
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %89
    Range %85-99
    1:4Average %98
    Range %91-104
    1:8Average %96
    Range %91-100
  • 回收率：
    The recovery of canine cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-108
    EDTA plasma (n=4)9590-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.245 2.250 2.248 2.071
    7501.716 1.704 1.710 1.533
    3751.144 1.161 1.153 0.976
    187.50.790 0.775 0.783 0.606
    940.459 0.474 0.467 0.290
    470.305 0.289 0.297 0.120
    23.50.215 0.211 0.213 0.036
    00.176 0.177 0.177 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能参考文献：
    1. No statistical differences were found between CRP and cTnI levels, either before or after surgical correction of brachycephaly in dogs. PMID: 25673910
    2. The objective of this study was to evaluate the correlation between anatomical components, clinical signs and several biomarkers, used to determine systemic inflammation and myocardial damage in dogs with brachycephalic upper airway obstructive syndrome. PMID: 22937913
    3. The use of NT-pro-BNP and troponin I levels as prognostic indicators for canine heart failure is reported. PMID: 22439324
    4. [NT-proBNP] concentration is significantly higher in dogs with pre-capillary pulmonary hypertension (PH) when compared to dogs with respiratory disease without PH; [NT-proANP] and [cTnI] were not elevated in dogs with pre-capillary PH. PMID: 21835711
    5. Moderate exercise does not present a confounding variable in the interpretation of cardiac tropinin I and C-reactive protein concentrations in normal dogs. PMID: 20840303
    6. Serum troponin I is high in dogs with either precapillary and postcapillary pulmonary hypertension (PH); myocardial damage in dogs with postcapillary PH is likely the consequence of increased mitral valve disease severity. PMID: 20002552
    7. Analysis of troponin I concentration has potential to increase knowledge of overall cardiac remodeling in dogs with mitral valve disease; the effect of age on troponin I levels should be considered in these patients. PMID: 20002554
    8. Circulating cTnI and pro-matrix metallopeptidase 9 increase in proportion to the severity of acute pulmonary thromboembolism PMID: 18047836
    9. The incidence of myocardial injury due to ehrlichiosis, as demonstrated by elevated cardiac troponin I blood levels, is reported. PMID: 18638021

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  • 蛋白家族：
    Troponin I family
  • 数据库链接：

    KEGG: cfa:403566

    STRING: 9615.ENSCAFP00000003805

    UniGene: Cfa.83