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Canine Luteinizing Hormone,LH ELISA Kit

  • 中文名称:
    犬黄体生成素(LH)酶联免疫试剂盒
  • 货号:
    CSB-E06790c
  • 规格:
    96T/48T
  • 价格:
    ¥4600/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Dog Luteinizing Hormone (LH) ELISA Kit was designed for the quantitative measurement of Dog Luteinizing Hormone (LH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.75 mIU/mL-60 mIU/mL and the sensitivity is 0.94 mIU/mL.

  • 别名:
    LHBLutropin subunit beta ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit; Lutropin beta chain ELISA Kit; Fragment ELISA Kit
  • 缩写:
    LH
  • Uniprot No.:
  • 种属:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.75 mIU/mL-60 mIU/mL
  • 灵敏度:
    0.94 mIU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of canine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 96  
    Range % 92-99  
    1:4 Average % 101  
    Range % 98-105  
    1:8 Average % 83  
    Range % 80-87  
  • 回收率:
    The recovery of canine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 84-92  
    EDTA plasma (n=4) 98 92-104  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    60 0.350 0.357 0.354    
    22.5 0.476 0.468 0.472    
    11.25 0.619 0.605 0.612    
    3.75 1.008 0.998 1.003    
    0.75 1.594 1.512 1.553    
    0 2.008 2.113 2.061    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Glycoprotein hormones subunit beta family
  • 数据库链接: