Canine Prostaglandin E2,PG-E2 ELISA Kit

产品详情

文献引用 (2) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Dog PG-E2 ELISA Kit was designed for the quantitative measurement of Dog PG-E2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 pg/mL-2000 pg/mL and the sensitivity is 5 pg/mL .
缩写：

PG-E2
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 pg/mL-2000 pg/mL
灵敏度：

5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-104
1:2	Average %	95
	Range %	89-105
1:4	Average %	93
	Range %	87-100
1:8	Average %	95
	Range %	90-101

回收率：

The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-96
EDTA plasma (n=4)	96	89-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.