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  4. Canine thymus activation regulated chemokine(TARC/CCL17)ELISA Kit

Canine thymus activation regulated chemokine(TARC/CCL17)ELISA Kit

  • 中文名称：
    犬胸腺活化调节趋化因子(TARC/CCL17)酶联免疫试剂盒
  • 货号：
    CSB-E17792c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Dog TARC/CCL17 ELISA Kit was designed for the quantitative measurement of Dog TARC/CCL17 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

  • 别名：
    CCL17 ELISA Kit; TARCC-C motif chemokine 17 ELISA Kit; CC chemokine TARC ELISA Kit; Small-inducible cytokine A17 ELISA Kit; Thymus and activation-regulated chemokine ELISA Kit
  • 缩写：
    TARC/CCL17
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.86 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %84-90
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %95
    Range %91-99
  • 回收率：
    The recovery of canine TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-99
    EDTA plasma (n=4)10097-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.908 2.005 1.957 1.801
    7501.179 1.205 1.192 1.036
    3750.734 0.738 0.736 0.580
    187.50.415 0.436 0.426 0.270
    940.311 0.328 0.320 0.164
    470.231 0.245 0.238 0.082
    23.50.198 0.189 0.194 0.038
    00.155 0.157 0.156
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Chemotactic factor for t lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4 and CCR8.
  • 基因功能参考文献：
    1. The present results suggest that TNF-alpha-induced CCL17 mRNA transcription in canine keratinocytes is positively regulated by p38 but negatively controlled by ERK. PMID: 20837364
    2. This study suggests that interferon-gamma may suppress granulocyte-macrophage colony stimulating factor production from canine keratinocytes, although further studies are required to confirm this. PMID: 20860556
    3. Inflammatory cytokines are important inducing factors for the production of CCL17 in the lesional skin of dogs with atopic dermatitis. atopic dermatitis. PMID: 20022349
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Expressed in thymus, spleen, lymph node, lung and heart.
  • 数据库链接：