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Chicken Heat Shock Protein 90,HSP-90 ELISA Kit

  • 中文名称：
    鸡热休克蛋白90(HSP-90)酶联免疫试剂盒
  • 货号：
    CSB-E12873C
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Chicken HSP90AA1 ELISA Kit was designed for the quantitative measurement of Chicken HSP90AA1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    HSP90AA1 ELISA Kit; HSPCAHeat shock protein HSP 90-alpha ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken HSP-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %85-103
    1:2Average %89
    Range %85-96
    1:4Average %93
    Range %83-99
    1:8Average %95
    Range %89-98
  • 回收率：
    The recovery of chicken HSP-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-101
    EDTA plasma (n=4)9587-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.428 2.388 2.408 2.303
    501.994 1.935 1.965 1.860
    251.362 1.334 1.348 1.243
    12.50.830 0.868 0.849 0.744
    6.250.618 0.585 0.602 0.497
    3.120.345 0.342 0.344 0.239
    1.560.251 0.262 0.257 0.152
    00.103 0.107 0.105
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from Hsp90 which acquires an open conformation for the next cycle.
  • 亚细胞定位：
    Cytoplasm. Melanosome.
  • 蛋白家族：
    Heat shock protein 90 family
  • 数据库链接：