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Chicken Insulin ELISA kit

  • 中文名称：
    鸡胰岛素（Insulin）酶联免疫试剂盒
  • 货号：
    CSB-E13293C
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Chicken INS ELISA Kit was designed for the quantitative measurement of Chicken INS protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 μIU/mL-50 μIU/mL and the sensitivity is 0.59 μIU/mL.

  • 别名：
    INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 μIU/mL-50 μIU/mL
  • 灵敏度：
    0.59 μIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken insulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-98  
    1:2 Average % 96  
    Range % 90-101  
    1:4 Average % 98  
    Range % 95-103  
    1:8 Average % 90  
    Range % 85-96  
  • 回收率：
    The recovery of chicken insulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-100  
    EDTA plasma (n=4) 97 95-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    50 0.141 0.145 0.143    
    25 0.310 0.313 0.312    
    12.5 0.685 0.676 0.681    
    6.25 1.477 1.472 1.475    
    3.12 2.709 2.707 2.708    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your ELISA kit CSB-E13293C, could you please advise on the following:
1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Does the standard include additive agent such as antiseptic or preservative? If it includes, what is the composition of said additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry.
CSB-E13293C
Standard：293 cell-derived recombinant protein.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
The kit doesn't contain hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 基因功能参考文献：
    1. Data suggest that insulin signaling in skeletal muscle can be regulated by dietary factors (here, dietary content of omega-3 fatty acids). PMID: 24094087
    2. Polymorphisms within the INS gene are associated with 55-week body weight. PMID: 16734694
    3. The present findings suggest the involvement of insulin in neuronal/glial coupling in the cerebellum. PMID: 19135128
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    KEGG: gga:396145

    STRING: 9031.ENSGALP00000010567

    UniGene: Gga.673