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  4. Chicken Interferon β,IFN-β/IFNB ELISA Kit

Chicken Interferon β,IFN-β/IFNB ELISA Kit

  • 中文名称：
    鸡β干扰素(IFN-β/IFNB)酶联免疫试剂盒
  • 货号：
    CSB-E11237Ch
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Chicken IFN-β/IFNB ELISA Kit was designed for the quantitative measurement of Chicken IFN-β/IFNB protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 pg/mL-200 pg/mL and the sensitivity is 3.125 pg/mL.

  • 别名：
    IFNB ELISA Kit; IFN2 ELISA Kit; Interferon type B ELISA Kit; IFN-beta ELISA Kit
  • 缩写：
    IFN-β/IFNB
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.5 pg/mL-200 pg/mL
  • 灵敏度：
    3.125 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 99  
    Range % 95-103  
    1:2 Average % 94  
    Range % 88-98  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 89  
    Range % 84-96  
  • 回收率：
    The recovery of chicken IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 88-94  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.342 0.341 0.342    
    75 0.494 0.486 0.490    
    37.5 0.652 0.664 0.658    
    12.5 1.067 1.076 1.072    
    2.5 1.478 1.575 1.527 　  
    0 1.854 1.955 1.905    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has antiviral activities.
  • 基因功能参考文献：
    1. Fpv012 inhibits induction of IFNB. PMID: 23427153
    2. RNA interference-mediated knockdown of ChMda5 showed that ChMda5 plays an important role in the IFN response of chicken cells to dsRNA PMID: 21444763
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 数据库链接：