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Chicken Interleukin 2,IL-2 ELISA Kit

  • 中文名称:
    鸡白介素2(IL-2)酶联免疫试剂盒
  • 货号:
    CSB-E06755Ch
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:
        IL-2 (Interleukin-2),是一种介导T细胞增殖和分化的白细胞介素,对于机体免疫功能的调节起着重要作用。IL-2具有促进T细胞增殖、增强T细胞活性、诱导自身再生及增殖等生物学功能,对于免疫细胞的分化和增殖调节至关重要。在某些肿瘤和自身免疫性疾病的治疗中,IL-2也有着广泛的应用。测定IL-2水平可作为评估患者的免疫功能状态的一种指标。
        j9九游会登录入口首页生物所提供的Chicken Interleukin 2,IL-2 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鸡血清、血浆、组织匀浆样本中的IL-2,其灵敏度为0.04 pg/mL,检测范围为0.18 pg/mL-12 pg/mL。
     
  • 缩写:
    IL-2
  • Uniprot No.:
  • 种属:
    Gallus gallus (Chicken)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.18 pg/mL-12 pg/mL
  • 灵敏度:
    0.04 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 85-96  
    1:2 Average % 90  
    Range % 85-97  
    1:4 Average % 99  
    Range % 94-106  
    1:8 Average % 97  
    Range % 92-104  
  • 回收率:
    The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 92-109  
    EDTA plasma (n=4) 96 90-104  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    12 2.151 2.198 2.175 2.072  
    6 1.835 1.869 1.852 1.749  
    3 1.457 1.487 1.472 1.369  
    1.5 1.098 1.051 1.075 0.972  
    0.75 0.698 0.682 0.690 0.587  
    0.38 0.449 0.460 0.455 0.352  
    0.18 0.236 0.222 0.229 0.126  
    0 0.102 0.104 0.103    
  • 数据处理:
  • 货期:
    3-5 working days

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  • 最新研究进展:
    IL-2是一种细胞因子,可刺激T细胞和自然杀伤细胞的增殖和活化。最新研究表明,IL-2在癌症和免疫治疗方面具有潜在的应用价值。例如,IL-2在肝癌和结直肠癌等方面的临床应用已经得到研究。此外,IL-2还被用于免疫治疗,如通过IL-2增强T细胞治疗肿瘤的方法。