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Dog Apolipoprotein A-I(APOA1) ELISA kit

  • 中文名称：
    犬载脂蛋白A1(Apo-A1)酶联免疫试剂盒
  • 货号：
    CSB-EL001913DO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog APOA1 ELISA Kit was designed for the quantitative measurement of Dog APOA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 28.12 ng/mL-1800 ng/mL and the sensitivity is 7 ng/mL.

  • 别名：
    APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    28.12 ng/mL-1800 ng/mL
  • 灵敏度：
    7 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %93
    Range %89-103
    1:2000Average %89
    Range %85-95
    1:4000Average %95
    Range %84-103
    1:8000Average %93
    Range %83-98
  • 回收率：
    The recovery of dog APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9392-95
    EDTA plasma (n=4)9591-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    18000.142 0.145 0.144
    9000.215 0.229 0.222
    4500.382 0.367 0.375
    2250.551 0.558 0.555
    112.50.973 0.990 0.982
    56.251.697 1.623 1.660
    28.122.287 2.328 2.308
    02.654 2.761 2.708
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 组织特异性：
    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
  • 数据库链接：