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Dog P-selectin(SELP) ELISA kit

  • 中文名称：
    犬P选择素(SELP)酶联免疫试剂盒
  • 货号：
    CSB-EL020978DO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog SELP ELISA Kit was designed for the quantitative measurement of Dog SELP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

  • 别名：
    RP1-86F14.2, CD62, CD62P, FLJ45155, GMP140, GRMP, LECAM3, PADGEM, PSEL, OTTHUMP00000032609|granulocyte membrane protein|leukocyte-endothelial cell adhesion molecule 3|platelet alpha-granule membrane
  • 缩写：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog SELP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 87-93
    1:2 Average % 104
    Range % 100-108
    1:4 Average % 86
    Range % 83-90
    1:8 Average % 105
    Range % 100-110
  • 回收率：
    The recovery of dog SELP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 84-93
    EDTA plasma (n=4) 102 98-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    800 2.584 2.468 2.526 2.402
    400 1.533 1.534 1.534 1.410
    200 0.985 0.990 0.988 0.864
    100 0.571 0.581 0.576 0.452
    50 0.365 0.346 0.356 0.232
    25 0.262 0.271 0.267 0.143
    12.5 0.194 0.196 0.195 0.071
    0 0.123 0.125 0.124 　
  • 数据处理：
  • 货期：
    3-5 working days

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