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  4. Dog Renin ELISA Kit

Dog Renin ELISA Kit

  • 中文名称：
    犬肾素(Renin)酶联免疫试剂盒
  • 货号：
    CSB-E08704c
  • 规格：
    96T/48T
  • 价格：
    ￥4800/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog REN ELISA Kit was designed for the quantitative measurement of Dog REN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    REN ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-97  
    1:2 Average % 105  
    Range % 100-112  
    1:4 Average % 84  
    Range % 80-88  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of dog renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 90-103  
    EDTA plasma (n=4) 88 84-93  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.582 2.683 2.633 2.454  
    2000 2.204 2.104 2.154 1.975  
    1000 1.451 1.521 1.486 1.307  
    500 0.864 0.908 0.886 0.707  
    250 0.569 0.584 0.577 0.398  
    125 0.430 0.402 0.416 0.237  
    62.5 0.279 0.259 0.269 0.090  
    0 0.182 0.176 0.179    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
  • 基因功能参考文献：
    1. With a moderate deficit in total body sodium and total body water, the renin-angiotensin-aldosterone system is the major factor controlling arterial blood pressure. PMID: 19930429
    2. Characterization of canine renin with mutations in the substrate binding site. PMID: 17942478
    3. Normotensive sodium loading in conscious dogs: regulation of renin secretion during beta1-receptor blockade. PMID: 19073902
  • 亚细胞定位：
    Secreted. Membrane.
  • 蛋白家族：
    Peptidase A1 family
  • 数据库链接：