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Dog gastrin (GAST) ELISA kit

  • 中文名称：
    犬胃泌素(GAST)酶联免疫试剂盒
  • 货号：
    CSB-EL009273DO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog GAST ELISA Kit was designed for the quantitative measurement of Dog GAST protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

  • 别名：
    GAST ELISA Kit; GASGastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
  • 缩写：
    GAST
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog GAST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-96
    1:2Average %97
    Range %92-102
    1:4Average %85
    Range %81-88
    1:8Average %89
    Range %83-96
  • 回收率：
    The recovery of dog GAST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-96
    EDTA plasma (n=4)8985-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4001.998 1.883 1.941 1.814
    2001.463 1.368 1.416 1.289
    1000.956 1.040 0.998 0.871
    500.623 0.655 0.639 0.512
    250.422 0.426 0.424 0.297
    12.50.287 0.270 0.279 0.152
    6.250.190 0.202 0.196 0.069
    00.132 0.122 0.127
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Gastrin/cholecystokinin family
  • 数据库链接：