Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Dog thrombomodulin,TM ELISA Kit

Dog thrombomodulin,TM ELISA Kit

  • 中文名称：
    犬血栓调节蛋白(TM)酶联免疫试剂盒
  • 货号：
    CSB-E06529c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog THBD ELISA Kit was designed for the quantitative measurement of Dog THBD protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

  • 别名：
    THBD ELISA Kit; Thrombomodulin ELISA Kit; TM ELISA Kit; CD antigen CD141 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.44 pg/mL-1500 pg/mL
  • 灵敏度：
    5.86 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:10Average %97
    Range %91-103
    1:20Average %94
    Range %91-98
    1:40Average %90
    Range %88-94
    1:80Average %93
    Range %89-97
  • 回收率：
    The recovery of dog TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8983-94
    EDTA plasma (n=4)9387-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    15000.087 0.086 0.087
    7500.148 0.142 0.145
    3750.234 0.229 0.232
    187.50.347 0.338 0.343
    93.750.562 0.576 0.569
    46.880.865 0.921 0.893
    23.441.464 1.392 1.428
    02.677 2.568 2.623
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • 基因功能参考文献：
    1. plasma levels are higher in dogs infected with Leishmania infantum PMID: 15372989
    2. cDNA sequence analysis, amino acid sequence homology, and tissue expression. PMID: 15585960
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in lung, liver, spleen, kidney, pancreas and lymph node. Low expression in heart, cerebrum, urinary bladder and uterus.
  • 数据库链接：