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  4. Fish Aromatase(ARO) ELISA Kit

Fish Aromatase(ARO) ELISA Kit

  • 中文名称：
    鱼芳香酶酶联免疫试剂盒
  • 货号：
    CSB-EL006394FI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    The Fish Aromatase (ARO) ELISA Kit is suitable for the quantitative detection of ARO in fish samples, including serum, plasma, and tissue homogenates. It has been validated with excellent sensitivity, high specificity, premium precision, high recovery, and lot-to-lot consistency. See the product instructions for more details.

    This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with goat-anti-rabbit ARO antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for ARO and HRP-conjugated ARO. The competitive inhibition reaction is launched between with HRP-labeled ARO and ARO in the samples or standards with the anti-fish ARO antibody. The substrate solution is added to the wells and the color develops in opposite to the amount of ARO in the sample. The color development is stopped and the intensity of the color is measured.

    ARO is the enzyme responsible for the aromatization of androgens to estrogen in many tissues, such as gonads, adipocytes, and the brain. It is important for sexual development. ARO helps make the estrogen that is conducive to fuel the growth of hormone-receptor-positive breast cancer. It may play a role in sex-determination. High levels of ARO was expressed at temperatures that yield female progeny in temperature-dependent sex determinant species. ARO also exerts a neuroprotective role. Many factors, including age, obesity, insulin, alcohol, and smoking, can enhance the activity of ARO.

  • 别名：
    cyp19a1 ELISA kit; cyp19Aromatase ELISA kit; EC 1.14.14.14 ELISA kit; CYPXIX ELISA kit; Cytochrome P-450AROM ELISA kit; Cytochrome P450 19A1 ELISA kit; Estrogen synthase ELISA kit
  • 缩写：
    CYP19A1
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    25 pg/mL-1000 pg/mL
  • 灵敏度：
    25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish ARO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 89-104    
    1:2 Average % 95    
    Range % 86-100    
    1:4 Average % 95    
    Range % 87-106    
    1:8 Average % 89    
    Range % 86-99    
  • 回收率：
    The recovery of fish ARO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 89-103    
    EDTA plasma (n=4) 94 90-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average 　    
    1000 0.154 0.156 0.155 　    
    500 0.359 0.355 0.357 　    
    230 0.573 0.564 0.569 　    
    75 0.877 0.862 0.870 　    
    25 1.561 1.583 1.572 　    
    0 2.342 2.369 2.356 　    
  • 本试剂盒所含材料：
    • A 96-well Assay plate--The 96-well plate has been pre-coated with goat-anti-rabbit ARO antibody.
    • Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-conjugated ARO (1 x 6 ml) --Bind to the anti-fish ARO antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Anti-fish ARO Antibody (1 x 6 ml)--React with both ARO in the samples/standards and HRP-conjugated ARO.
    • Wash Buffer (20x concentrate) (1 x 15ml) --Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --Mix with Substrate B and interacts with HRP, eliciting the solution turns blue.
    • Substrate B (1 x 7 ml) --Mix with Substrate A and interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Per customers request, please provide the following information regarding Fish Aromatase, ARO ELISA Kit CSB-EL006394FI
1) What sample types have been tested with this kit.
2) What observed concentrations were obtained from these sample types and what dilutions were used
3) Please provide the QC data for sample tested with this kit.
4) Is the capture antibody a monoclonal or polyclonal?
5) What is the host of the capture antibody?
6) What’s the immunogen or immunogen range of the capture antibody?
7) What’s is the species of the immunogen of the capture antibody, human or something else?
8) Is the detection antibody a monoclonal or polyclonal?
9) What is the host of the detection antibody?
10) What is the immunogen or immunogen range of the detection antibody?
11) What is the species of the immunogen of the detection antibody? Human or something else?
12) What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
13) If recombinant protein, is it full length or partial?
14) What is the species of the standard– human or something else?

A:
Thanks for your inquiry!
CSB-EL006394FI
This is a competitive kit and there is only one antibody in the kit.
The plate is coated with rabbit polyclonal antibody.
Immunogen is 293 cell-derived recombinant protein. It is affinity purified.
Standard is 293 cell-derived recombinant protein.
This kit is designed according to: https://www.uniprot.org/uniprot/Q92087.
We tested undiluted serum and plasma samples before and the test value is 25--75pg/ml.
Pls find attached QC.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 亚细胞定位：
    Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：