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Fish Insulin-like growth factor II(IGF2) ELISA kit

  • 中文名称：
    鱼胰岛素样生长因子II(IGF2)酶联免疫试剂盒
  • 货号：
    CSB-EL011088FI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish IGF2 ELISA Kit was designed for the quantitative measurement of Fish IGF2 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 别名：
    igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma
  • 检测范围：
    62.5 pg/mL-1000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 91
    Range % 87-102
    1:200 Average % 95
    Range % 91-99
    1:400 Average % 96
    Range % 88-106
    1:800 Average % 92
    Range % 88-96
  • 回收率：
    The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 90-102
    EDTA plasma (n=4) 93 85-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　
    1000 0.085 0.089 0.087 　
    500 0.192 0.205 0.199 　
    250 0.485 0.489 0.487 　
    125 0.942 0.937 0.940 　
    62.5 1.613 1.572 1.593 　
    0 2.595 2.624 2.610 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for integrin which is required for IGF2 signaling.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    UniGene: Omy.11849