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  4. Fish Interleukin 6,IL-6 ELISA Kit

Fish Interleukin 6,IL-6 ELISA Kit

  • 中文名称：
    鱼白介素6(IL-6)酶联免疫试剂盒
  • 货号：
    CSB-E13258Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish IL-6 ELISA Kit was designed for the quantitative measurement of Fish IL-6 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-200 pg/mL and the sensitivity is 12.5 pg/mL.

  • 别名：
    il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 缩写：
    IL-6
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-200 pg/mL
  • 灵敏度：
    12.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-97  
    1:2 Average % 89  
    Range % 82-94  
    1:4 Average % 103  
    Range % 94-107  
    1:8 Average % 96  
    Range % 90-101  
  • 回收率：
    The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 91-104  
    EDTA plasma (n=4) 91 84-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.128 0.133 0.131    
    100 0.269 0.258 0.264    
    50 0.449 0.431 0.440    
    25 0.851 0.842 0.847    
    12.5 1.813 1.783 1.798    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 组织特异性：
    Expressed in kidney and spleen. Low expression in liver and gills.