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  4. Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

  • 中文名称：
    鱼肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
  • 货号：
    CSB-E13254Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish TNF-α ELISA Kit was designed for the quantitative measurement of Fish TNF-α protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-5000 pg/mL and the sensitivity is 125 pg/mL.

  • 缩写：
    TNF-α
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma
  • 检测范围：
    125 pg/mL-5000 pg/mL
  • 灵敏度：
    125 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 89-100  
    1:2 Average % 93  
    Range % 87-99  
    1:4 Average % 95  
    Range % 88-103  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-102  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    5000 0.154 0.156 0.155    
    2500 0.351 0.328 0.340    
    1150 0.562 0.535 0.549    
    375 0.861 0.864 0.863    
    125 1.544 1.530 1.537    
    0 2.387 2.355 2.371    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
  • 亚细胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Tumor necrosis factor family