Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Goat Haptoglobin,Hpt/HP ELISA KIT

Goat Haptoglobin,Hpt/HP ELISA KIT

  • 中文名称：
    山羊结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒
  • 货号：
    CSB-E13210G
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Goat Hpt/HP ELISA Kit was designed for the quantitative measurement of Goat Hpt/HP protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 3.9 ng/mL-1000 ng/mL and the sensitivity is 1.95 ng/mL.

  • 缩写：
    Hpt/HP
  • 种属：
    Capra hircus (Goat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.9 ng/mL-1000 ng/mL
  • 灵敏度：
    1.95 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat haptoglobin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 84-99  
    1:200 Average % 95  
    Range % 90-101  
    1:400 Average % 97  
    Range % 87-101  
    1:800 Average % 94  
    Range % 88-98  
  • 回收率：
    The recovery of goat haptoglobin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 96 91-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1000 0.311 0.313 0.312    
    250 0.573 0.586 0.580    
    62.5 0.935 0.948 0.942    
    15.6 1.581 1.596 1.589    
    3.9 1.986 2.005 1.996    
    0 2.182 2.236 2.209    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:


I have a doubt Optical density and concentration of a compound under study is directly correlated, isn't it?

A:
Thanks for your inquiry.
Different elisa kits might have different curves.Let us see the examples of kits which employes sanwitch method and competition method.They both have OD values as X-axis and the concentraction of antigen as Y-axis.
For kits of sanwitch method,the concentration of antigen is directly proportional to the OD value.In the detection range of the kit,OD values increase when concentration of antigen increases。For the kits of competition method,you can refer to the manual of the kits.In the dtection range of the kit,the OD values decrease with increase of antigen concentration.Thus the pics on the web is normal and with no problems.Regarding the mentioned kit#CSB-E13210G,Goat Haptoglobin,Hpt/HP ELISA KIT, competitive inhibition enzyme immunoassay technique is employeed.The OD value of samples is inversely proportional to the concentration.It developes deeper color,it will have higher OD value and lower concentration. Pls let me know if you have any further questions. Thank you.
Q:


In this mannual, 1:500 dilution is there..I need 1:50000 dilution for getting d result
How can I achieve that 03:35:01 pm
R u available? 03:37:09 pm
I need 1:50000 dilution of sample instead of 1:500 given in the mannual for getting result..How can I achieve that and is there any problem in that dilution? 03:38:53 pm
And if so I need more diluent. may I know composition of diluent or whether may I get extra diluent.No colour means max concentration of 1000ng/ml..isnt it?

A:
The dilution factor 1:500 is a recommanded diltuion factor.We suggest you use it as a baseline and do the pre-testing first and confirm the best dilution factor for your own test.Usually the dilution factor you will obtain from the pre-testing is usually not too far away from the recommanded diltuion factor.We are sorry the the specific information of the sample diluent is confidential and we are not able to disclosed it.
May I know why you need 1:50000?What results do you need to get by 1:50000?
No colour means max concentration of 1000ng/ml..isnt it?---As to this question about the color,we could not inform you a general conclustion.It needs to be based on specific situation.For example,how is the "no color" caused?