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  4. Goat Immunoglobulin M(IgM) ELISA Kit

Goat Immunoglobulin M(IgM) ELISA Kit

  • 中文名称：
    山羊免疫球蛋白M(IgM)酶联免疫试剂盒
  • 货号：
    CSB-E13134G
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Goat IgM ELISA Kit was designed for the quantitative measurement of Goat IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 24.4 ng/mL-6250 ng/mL and the sensitivity is 24.4 ng/mL.

  • 缩写：
    IgM
  • 种属：
    Capra hircus (Goat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    24.4 ng/mL-6250 ng/mL
  • 灵敏度：
    24.4 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5000 Average % 94    
    Range % 89-103    
    1:10000 Average % 89    
    Range % 81-99    
    1:20000 Average % 97    
    Range % 89-105    
    1:40000 Average % 95    
    Range % 83-98    
  • 回收率：
    The recovery of goat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 82-105    
    EDTA plasma (n=4) 95 88-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    6250 0.379 0.383 0.381      
    1562.5 0.736 0.742 0.739      
    390.6 1.522 1.508 1.515      
    97.6 2.246 2.219 2.233      
    24.4 2.548 2.569 2.559      
    0 2.756 2.783 2.77      
  • 数据处理：
  • 货期：
    3-5 working days

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