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  4. Guinea pig interleukin 2,IL-2 ELISA Kit

Guinea pig interleukin 2,IL-2 ELISA Kit

  • 中文名称：
    豚鼠白介素2(IL-2)酶联免疫试剂盒
  • 货号：
    CSB-E12914Gp
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

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  • 产品描述：

    The Guinea pig interleukin 2 (IL-2) ELISA Kit is a powerful tool for researchers in the field of immunology. The kit is designed for quantitative measurement of interleukin-2 protein in samples such as serum, plasma, and tissue homogenates of Cavia porcellus (Guinea pig).

    The kit offers a broad detection range of 31.25 pg/mL-2000 pg/mL and a high sensitivity of 7.8 pg/mL, enabling accurate and reliable measurement of IL-2 levels in even low-concentration samples. With an assay time of 1-5 hours and a sample volume requirement of only 50-100ul, our kit is efficient and easy to use.

    This Guinea pig IL-2 ELISA Kit employs a sandwich assay principle, where the target IL-2 protein is captured by a specific antibody and detected by a second antibody conjugated to a substrate. And the detection wavelength is 450 nm.

  • 缩写：
    IL-2
  • Uniprot No.：
  • 种属：
    Guinea Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %102
    Range %98-108
    1:2Average %94
    Range %91-98
    1:4Average %103
    Range %97-108
    1:8Average %88
    Range %80-96
  • 回收率：
    The recovery of guinea pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-100
    EDTA plasma (n=4)10496-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.230 2.351 2.291 2.152
    10001.713 1.794 1.754 1.615
    5001.214 1.235 1.225 1.086
    2500.743 0.732 0.738 0.599
    1250.495 0.464 0.480 0.341
    62.50.349 0.338 0.344 0.205
    31.250.255 0.251 0.253 0.114
    00.139 0.138 0.139 　
  • 数据处理：
  • 货期：
    3-5 working days

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