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Horse insulin (INS) ELISA kit

  • 中文名称：
    马胰岛素(INS)酶联免疫试剂盒
  • 货号：
    CSB-EL011742HO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Horse INS ELISA Kit was designed for the quantitative measurement of Horse INS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 nIU/mL-100 nIU/mL and the sensitivity is 0.39 nIU/mL.

  • 别名：
    INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Equus caballus (Horse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 nIU/mL-100 nIU/mL
  • 灵敏度：
    0.39 nIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of horse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %99
    Range %96-102
    1:2000Average %87
    Range %84-91
    1:4000Average %95
    Range %90-99
    1:8000Average %98
    Range %94-102
  • 回收率：
    The recovery of horse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-92
    EDTA plasma (n=4)104100-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nIU/mlOD1OD2AverageCorrected
    1002.659 2.698 2.679 2.546
    502.353 2.301 2.327 2.194
    251.922 1.904 1.913 1.780
    12.51.179 1.198 1.189 1.056
    6.250.702 0.732 0.717 0.584
    3.120.411 0.435 0.423 0.290
    1.560.305 0.330 0.318 0.185
    00.132 0.134 0.133
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family