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Horse insulin-like growth factor 1 (IGF1) ELISA kit

  • 中文名称：
    马胰岛素样生长因子1(IGF1)酶联免疫试剂盒
  • 货号：
    CSB-EL011086HO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Horse IGF1 ELISA Kit was designed for the quantitative measurement of Horse IGF1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

  • 别名：
    IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit; Fragment ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Equus caballus (Horse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of horse IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %89-95
    1:2Average %95
    Range %90-104
    1:4Average %92
    Range %87-100
    1:8Average %89
    Range %80-97
  • 回收率：
    The recovery of horse IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9587-101
    EDTA plasma (n=4)8782-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8001.961 1.944 1.953 1.820
    4001.488 1.605 1.547 1.414
    2001.178 1.213 1.196 1.063
    1000.912 0.902 0.907 0.774
    500.669 0.657 0.663 0.530
    250.405 0.396 0.401 0.268
    12.50.260 0.249 0.255 0.122
    00.137 0.129 0.133
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：