Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human α-Glucosidase ELISA Kit

Human α-Glucosidase ELISA Kit

  • 中文名称：
    人α葡萄糖苷酶(α-glucosidase)酶联免疫试剂盒
  • 货号：
    CSB-E09459h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MGAM ELISA Kit was designed for the quantitative measurement of Human MGAM protein in serum, plasma, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 0.78 μU/mL.

  • 别名：
    4-alpha-glucosidase ELISA Kit; Alpha-glucosidase ELISA Kit; Glucan 1 ELISA Kit; Glucoamylase ELISA Kit; Maltase-glucoamylase; intestinal ELISA Kit; MGA ELISA Kit; MGA_HUMAN ELISA Kit; MGAM ELISA Kit; MGAML ELISA Kit
  • 缩写：
    MGAM
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, urine
  • 检测范围：
    3.12 μU/mL-200 μU/mL
  • 灵敏度：
    0.78 μU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human α-Glucosidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 95
    Range % 85-103
    1:40 Average % 90
    Range % 85-99
    1:80 Average % 95
    Range % 91-102
    1:100 Average % 89
    Range % 81-97
  • 回收率：
    The recovery of human α-Glucosidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 85-101
    EDTA plasma (n=4) 93 87-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µU/ml OD1 OD2 Average Corrected
    200 2.127 2.112 2.120 1.913
    100 1.746 1.757 1.752 1.545
    50 1.442 1.417 1.430 1.223
    25 1.069 1.028 1.049 0.842
    12.5 0.835 0.843 0.839 0.632
    6.25 0.502 0.521 0.512 0.305
    3.12 0.347 0.354 0.351 0.144
    0 0.205 0.209 0.207 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing.
  • 基因功能参考文献：
    1. Mechanistic Pathway on Human alpha-Glucosidase Maltase-Glucoamylase Unveiled by QM/MM Calculations PMID: 29548257
    2. MGAM, or nearby regulatory elements, may be involved in the etiology of oral clefts. PMID: 25776870
    3. Starch internal structure modulates its susceptibility to MGAM. The internal branch amounts negatively affect the glucose release rate. PMID: 25037326
    4. Findings suggest that C-terminal subunits of recombinant maltase-glucoamylase (MGAM) assists alpha-amylase in digesting starch molecules and potentially may compensate for developmental or pathological amylase deficiencies. PMID: 22563462
    5. These results suggest that the N-terminal and C-terminal catalytic domains of maltase-glucoamylase differ in their substrate specificities and inhibitor tolerance despite their structural relationship PMID: 22036121
    6. we report crystal structures of C-terminal maltase-glucoamylase alone at a resolution of 3.1 angstroms, and in complex with its inhibitor acarbose PMID: 22058037
    7. analysis of substrate selectivity of human maltase-glucoamylase and sucrase-isomaltase N-terminal domains PMID: 20356844
    8. genetic analysis of MGAM, exon boundaries, and chromosome mapping PMID: 12547908
    9. Raw starch granule degradation with recombinanat human MGAM indicates that pancreatic alpha-amylase hydrolysis is not a requirement for native starch digestion in the human small intestine. PMID: 17485087
    10. Intestinal maltase-glycoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity. PMID: 18036614
    11. Acarbose has been found to improve insulin levels and thus glucose/insulin ratios more effectively in overweight patients compared with nonoverweight patients with PCOS. PMID: 18377903
    12. This study reported the first diagnosed Finnish patient with a phenotype compatible with the late-onset form of Pompe disease. Molecular genetic analysis of the GAA gene revealed a novel missense mutation (Y575X),combined with (P545L) mutation. PMID: 19472353

    显示更多

    收起更多

  • 亚细胞定位：
    Apical cell membrane; Single-pass type II membrane protein. Note=Brush border.
  • 蛋白家族：
    Glycosyl hydrolase 31 family
  • 组织特异性：
    Expressed in small intestine, granulocyte, and kidney but not in salivary gland or pancreas.
  • 数据库链接：

    HGNC: 7043

    OMIM: 154360

    KEGG: hsa:8972

    STRING: 9606.ENSP00000447378

    UniGene: Hs.122785