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  4. Human 26S proteasome non-ATPase regulatory subunit 4(PSMD4) ELISA kit

Human 26S proteasome non-ATPase regulatory subunit 4(PSMD4) ELISA kit

  • 中文名称：
    人26S蛋白酶体非ATP酶调节亚基4(PSMD4)酶联免疫试剂盒
  • 货号：
    CSB-EL018908HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PSMD4 ELISA Kit was designed for the quantitative measurement of Human PSMD4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 34.4 pg/mL-2200 pg/mL and the sensitivity is 8.6 pg/mL.

  • 别名：
    26S protease subunit S5a ELISA Kit; 26S proteasome non ATPase regulatory subunit 4 ELISA Kit; 26S proteasome non-ATPase regulatory subunit 4 ELISA Kit; 26S proteasome regulatory subunit rpn10 ELISA Kit; 26S proteasome regulatory subunit S5A ELISA Kit; AF 1 ELISA Kit; AF ELISA Kit; AF1 ELISA Kit; Angiocidin ELISA Kit; Antisecretory factor 1 ELISA Kit; ASF ELISA Kit; DS5a ELISA Kit; MCB 1 ELISA Kit; MCB1 ELISA Kit; Multiubiquitin chain binding protein ELISA Kit; Multiubiquitin chain-binding protein ELISA Kit; OTTHUMP00000059963 ELISA Kit; Prosome macropain ELISA Kit; Proteasome (prosome macropain) 26S subunit non ATPase 4 ELISA Kit; Proteasome 19S S5A ELISA Kit; Proteasome 26S non ATPase subunit 4 ELISA Kit; Proteasome 26S subunit non ATPase 4 ELISA Kit; PSMD 4 ELISA Kit; Psmd4 ELISA Kit; PSMD4_HUMAN ELISA Kit; pUB R5 ELISA Kit; pUBR5 ELISA Kit; Rpn 10 ELISA Kit; Rpn10 ELISA Kit; RPN10 homolog ELISA Kit; S5A ELISA Kit; S5a/antisecretory factor protein ELISA Kit
  • 缩写：
    PSMD4
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    34.4 pg/mL-2200 pg/mL
  • 灵敏度：
    8.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PSMD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 84-94    
    1:2 Average % 102    
    Range % 97-107    
    1:4 Average % 98    
    Range % 88-102    
    1:8 Average % 99    
    Range % 92-104    
  • 回收率：
    The recovery of human PSMD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 91-102    
    EDTA plasma (n=4) 95 88-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2200 2.302 2.234 2.268 2.120    
    1100 1.560 1.488 1.524 1.376    
    550 0.901 0.932 0.917 0.769    
    275 0.502 0.511 0.507 0.359    
    137.5 0.293 0.287 0.290 0.142    
    68.8 0.249 0.246 0.248 0.100    
    34.4 0.196 0.190 0.193 0.045    
    0 0.152 0.144 0.148 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
  • 基因功能参考文献：
    1. Preterm delivery is associated with low levels of anti-secretory factor in placenta. Inflammation, a potential trigger of preterm birth, is more pronounced in the preterm placenta and inversely related to the placental level of anti-secretory factor. PMID: 29265188
    2. We therefore suggest that PSMD4 or b-catenin might be potential targets for suppressing tumor aggressiveness, and consequently, improving outcomes in patients whose tumors express cNrf2. PMID: 27033953
    3. Binding (especially high-affinity binding) of non-ubiquitinated proteins to the Rpn10 proteasome subunit can both regulate the functioning of this proteasomal ubiquitin receptor (by competing with ubiquitinated substrates) and promote activation of other pathways for proteolytic degradation of proteins destined to the proteasome. PMID: 28988533
    4. The platelet 26S proteasome exhibits different basal activities of its catalytic subunits and chymotrypsin-like activity is most prominently enhanced by calcium dependent signaling. Collagen stimulation enhances 26S proteasome chymotrypsin-like activity in platelets. PMID: 27768934
    5. regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. PMID: 26903513
    6. Not only AF1, but an entire proteasome complex, seems to be present in blood. PMID: 25897558
    7. binds to death receptor-6, and induces monocyte cell line differentiation via NF-kB pathway PMID: 24829148
    8. The VWA domain regulation of ubiquitin and Ubl binding to S5a is restricted to the 26S proteasome. PMID: 25318673
    9. The degradation of TP53 and MDM2 by the proteasome can be selectively dependent on S5a in human cells. PMID: 24121268
    10. To examine angiocidin expression in SMMC-7221 and HepG2 cells and the role of angiocidin in liver cancer cell growth. Angiocidin is highly expressed in liver cancer cells, and it may play a key role in tumor growth of liver cancers. PMID: 24250289
    11. s demonstrate that human cytomegalovirus UL76 induces a novel nuclear aggresome, likely by subverting S5a of the ubiquitin-proteasome system. PMID: 23966401
    12. One consequence of the interaction between E6/E6AP and S5a is enhanced ubiquitination of this proteasome subunit. PMID: 24074603
    13. These studies suggest that diminished 26S activity in failing human hearts may be related ti impaired docking of the 19S to the 20S as a result of decreased Rpt subunit ATPase activity and alpha7 subunit phosphorylation. PMID: 23515276
    14. identified the VWA domain of hRpn10 as a receptor for ubiquitin-like proteins within the 26S proteasome and elucidated how FAT10 mediates efficient proteolysis by the proteasome PMID: 22434192
    15. Both higher PSMD4 expression levels and higher 1q21 copy numbers affected clinical outcome adversely. PMID: 21628408
    16. results suggest that there is different substrate specificity between S5a and hRpn13 at the level of delivery and S5a may be the major docking site for ERAD substrates. PMID: 20417181
    17. Overexpression of the S5a subunit of proteasome activator PA700 did not recover proteasome function in Huntington disease cells. S5a. PMID: 17327906
    18. parkin Ubld uses differential surfaces to recruit UIM regions from the S5a proteasomal subunit compared with Eps15 involved in cell signaling. PMID: 19875440
    19. S5a component of the 19S complex interacts with different ubiquitin-like (ubl) modules PMID: 11827521
    20. HHr23a binds to this protein, which changes its own conformation. PMID: 14557549
    21. structure of the ubiquitin-interacting motif of the proteasome subunit S5a bound to the ubiquitin-like domain of HR23B PMID: 14585839
    22. Results report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules, and present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin. PMID: 15826667
    23. These results are consistent with the conclusion that the anti-tumor activity of angiocidin arises from its ability to ligate collagen and alpha2beta1 on endothelial cells and tumor cells. PMID: 16762342
    24. UIM2 domain of S5a binds preferentially to hHR23a over polyubiquitin, and a model is provided for the ternary complex that represents one of the mechanisms used by the proteasome to capture ubiquitylated substrates. PMID: 17408689
    25. results suggest that S5a is regulated during apoptosis at the transcriptional level and S5a upregulation by antiapoptotic signals can contribute to cell survival. PMID: 17459097
    26. S5a-UIMs can be used as upstream inhibitors of the proteasome pathway. PMID: 17949686
    27. In autoimmune disorders like MS, angiocidin activates T cells, macrophages, microglia & astrocytes to produce inflammatory cytokines and to stimulate antigen presentation. PMID: 18207252
    28. angiocidin activates monocytes to secrete cytokines and differentiates them to a macrophage-like phenotype through at least two pathways mediated by MAPK and NF-kappaB, as well as PI3K PMID: 18632645
    29. The presence of S5a in the reaction containing a substrate (luciferase), ubiquitination enzymes (an E2, UbcH5, and the U-box E3 ligase CHIP) and 26S proteasome significantly increased the rate of luciferase degradation. PMID: 19387488
    30. Angiocidin's ability to elicit tumor cell death may be mediated in part by it's pro-inflammatory effects on immune cells in the tumor microenvironment. PMID: 19519434
    31. The binding of S5a to K48-linked diubiquitin is defined at an atomic level resolution. PMID: 19683493
    32. Proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (also called subunit 5a) has two independent polyubiquitin binding sites whose sequences are highly conserved among higher eukaryotic orthologs. PMID: 9488668

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  • 蛋白家族：
    Proteasome subunit S5A family
  • 数据库链接：

    HGNC: 9561

    OMIM: 601648

    KEGG: hsa:5710

    STRING: 9606.ENSP00000357879

    UniGene: Hs.505059