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中文名称:人6-羟基硫酸褪黑素(6HMS)酶联免疫试剂盒
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货号:CSB-EQ027870HU
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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缩写:6HMS
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates
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检测范围:10 pg/mL- 400 pg/mL
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灵敏度:10 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Others
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human 6HMS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 92-98 1:2 Average % 95 Range % 87-105 1:4 Average % 96 Range % 89-102 1:8 Average % 92 Range % 88-96 -
回收率:
The recovery of human 6HMS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 89-100 EDTA plasma (n=4) 95 91-101 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 400 0.154 0.156 0.155 200 0.360 0.342 0.351 92 0.572 0.543 0.558 30 0.856 0.860 0.858 10 1.475 1.489 1.482 0 2.341 2.435 2.388 -
数据处理:
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货期:3-5 working days
引用文献
- Modulation of aqueous humor melatonin levels by yellow-filter and its protective effect on lens VE Lledó,Journal of Photochemistry and Photobiology B: Biology,2021
- Melatonin as a potential therapy for sepsis: a phase I dose escalation study and an ex vivo whole blood model under conditions of sepsis Galley HF et al,J Pineal Res,2014
问答及客户评论
I would like to know if you recommend using lysis buffer in order to remove the blood from the tissue when running the 6-hydroxymelatonin ELISA KIT in tissue. Our specimens are largely supplied by blood what could mask results.
So sorry that we have no experience on using lysis buffer remove the blood from the tissue.
Anyway,if it helps,we are glad to recommend to you our sample method on tissue stated it as in the manual"100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C"
And if you worry the blood effect, pls use PBS to wash the sample longer.
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