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  4. Human A disintegrin and metalloproteinase with thrombospondin motifs 2(ADAMTS2) ELISA kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 2(ADAMTS2) ELISA kit

  • 中文名称：
    人金属肽酶含血小板反应蛋白2(ADAMTS2)酶联免疫试剂盒
  • 货号：
    CSB-EL001308HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ADAMTS2 ELISA Kit was designed for the quantitative measurement of Human ADAMTS2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 别名：
    A disintegrin and metalloproteinase with thrombospondin motifs 2 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 2 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 2 ELISA Kit; ADAM TS 2 ELISA Kit; ADAM TS2 ELISA Kit; ADAM-TS 2 ELISA Kit; ADAM-TS2 ELISA Kit; ADAMTS 3 ELISA Kit; ADAMTS-2 ELISA Kit; ADAMTS2 ELISA Kit; ATS2_HUMAN ELISA Kit; EC 3.4.24.14 ELISA Kit; EDS VIIB ELISA Kit; EDS VIIC ELISA Kit; hPCPNI ELISA Kit; NPI ELISA Kit; PC I NP ELISA Kit; PC I-NP ELISA Kit; PCINP ELISA Kit; PCPNI ELISA Kit; pNPI ELISA Kit; Procollagen I N proteinase ELISA Kit; Procollagen I N-proteinase ELISA Kit; Procollagen I/II amino propeptide processing enzyme ELISA Kit; Procollagen I/II amino propeptide-processing enzyme ELISA Kit; Procollagen N endopeptidase ELISA Kit; Procollagen N-endopeptidase ELISA Kit
  • 缩写：
    ADAMTS2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-95
    1:2 Average % 92
    Range % 88-96
    1:4 Average % 87
    Range % 82-91
    1:8 Average % 95
    Range % 90-100
  • 回收率：
    The recovery of human ADAMTS2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 98-109
    EDTA plasma (n=4) 97 92-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.129 2.238 2.184 2.023
    100 1.737 1.682 1.710 1.549
    50 1.112 1.174 1.143 0.982
    25 0.719 0.741 0.730 0.569
    12.5 0.480 0.459 0.470 0.309
    6.25 0.360 0.350 0.355 0.194
    3.12 0.239 0.247 0.243 0.082
    0 0.159 0.163 0.161 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form with reduced collagen-binding activity.
  • 基因功能参考文献：
    1. We identified three novel homozygous loss-of-function mutations (c.2927_2928delCT, p.(Pro976Argfs*42); c.669_670dupG, p.(Pro224Argfs*24); and c.2751-2A>T) and one compound heterozygous mutation (c.2T>C, p.? and c.884_887delTGAA, p.(Met295Thrfs26*)) in ADAMTS2 in five patients from four unrelated families PMID: 26765342
    2. Data indicate that ADAMTS2 and 3 cleave the amino-propeptide of fibrillar collagens and regulate blood vessels homeostasis and lymphangiogenesis. Also, ADAMTS2 deficiency leads to the dermatosparactic type of Ehlers-Danlos syndrome. [review] PMID: 25863161
    3. IL-1a is a strong positive regulator of ADAMTS-2 and ADAMTS-3 expression. PMID: 26232334
    4. ADAMTS2 was significantly overexpressed in Fibrous dysplasia (FD) tissues, but rarely expressed in normal bone tissues, suggesting that ADAMTS2 could be a potential biomarker for FD PMID: 25674217
    5. Pathway inhibition studies revealed that ADAMTS-2 upregulation by IL-6 was mediated by JNK pathway. PMID: 24752352
    6. Data show that ADAMTS-2 is able to reduce proliferation of endothelial cells, and to induce their retraction and detachment from the substrate resulting in apoptosis. PMID: 20574651
    7. Determination of the processing, activity and cleavage specificity of the bovine ADAMTS2 protein. PMID: 16046392
    8. when conditioned media of TSP2-transfectants were added to cultures of bovine pulmonary microvascular endothelial cells (BPMEC), the BPMEC proliferation was significantly inhibited; suggesting that human TSP2 is a potential inhibitor of angiogenesis PMID: 11788898
    9. Although exons 3-5 or 14-16 encode protein domains that have not been recognized as crucial for ADAMTS-2 activity, the aminoprocollagen processing was strongly impaired, providing evidence for the requirement of these domains for proper enzyme function PMID: 15373769

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  • 相关疾病：
    Ehlers-Danlos syndrome 7C (EDS7C)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain.
  • 数据库链接：

    HGNC: 218

    OMIM: 225410

    KEGG: hsa:9509

    STRING: 9606.ENSP00000251582

    UniGene: Hs.23871