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  4. Human A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit

  • 中文名称：
    人金属肽酶含血小板反应蛋白5(ADAMTS5)酶联免疫试剂盒
  • 货号：
    CSB-EL001312HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The product CSB-EL001312HU is a sandwich ELISA kit developed to measure levels of human ADAMTS5 in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of ADAMTS5 bound in the initial step.

    ADAMTS5 is a key enzyme in osteoarthritis (OA) by degrading aggrecan as an aggrecanase. Since aggrecan destruction in synovial fluid is a hallmark at the early stage of OA, the major aggrecanase, ADAMTS5, is identified as a potential biomarker for the prediction of OA progression. It also plays important roles in normal physiology as well as pathologies such as cancer. ADAMTS5's roles in cancer vary from different types. Downregulation or hypermethylation of ADAMTS5 suppressed its tumor suppressor function in mouse melanoma, human hepatocellular carcinoma (HCC), and human colorectal cancer. Overexpression of ADAMTS5 has also been reported in human glioblastomas and stimulates cell invasion.

  • 别名：
    A disintegrin and metalloproteinase with thrombospondin motifs 11 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 5 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) ELISA Kit; A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 5 ELISA Kit; A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 5 ELISA Kit; ADAM TS 11 ELISA Kit; ADAM TS 5 ELISA Kit; ADAM TS5 ELISA Kit; ADAM-TS 11 ELISA Kit; ADAM-TS 5 ELISA Kit; ADAM-TS5 ELISA Kit; ADAMTS 11 ELISA Kit; ADAMTS 5 ELISA Kit; ADAMTS-11 ELISA Kit; ADAMTS-5 ELISA Kit; ADAMTS11 ELISA Kit; ADAMTS11, formerly ELISA Kit; Adamts5 ELISA Kit; ADMP 2 ELISA Kit; ADMP-2 ELISA Kit; ADMP2 ELISA Kit; Aggrecanase 2 ELISA Kit; Aggrecanase-2 ELISA Kit; ATS5_HUMAN ELISA Kit; FLJ36738 ELISA Kit; Implantin ELISA Kit; ThromboSpondin motif-5 ELISA Kit
  • 缩写：
    ADAMTS5
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-94  
    1:2 Average % 104  
    Range % 100-107  
    1:4 Average % 92  
    Range % 87-95  
    1:8 Average % 98  
    Range % 93-101  
  • 回收率：
    The recovery of human ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-99  
    EDTA plasma (n=4) 89 82-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.154 2.254 2.204 2.062  
    500 1.512 1.612 1.562 1.420  
    250 1.001 1.011 1.006 0.864  
    125 0.536 0.546 0.541 0.399  
    62.5 0.328 0.328 0.328 0.186  
    31.25 0.257 0.267 0.262 0.120  
    15.6 0.189 0.199 0.194 0.052  
    0 0.142 0.141 0.142 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. Participates in development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
  • 基因功能参考文献：
    1. Data suggest that 3prime untranslated region of ADAMTS5 mRNA contains 'seedmatched-sequence' for hsa-miR-140-3p (microRNA-140-3p); in chondrocytes from articular cartilage of patients with knee osteoarthritis, interleukin-1beta up-regulates expression of ADAMTS5 and down-regulates expression of hsa-miR-140-3p. (ADAMTS5 = ADAM metallopeptidase with thrombospondin type 1 motif 5 A) PMID: 28110479
    2. A novel genetic variant in ADAMTS5 is associated with bicuspid aortic valve disease. PMID: 29162281
    3. expression by synovial cells induced by hemoglobin at low doses, suggesting a possible role for hemoglobin in cartilage damage after intra-articular hemorrhage PMID: 29137610
    4. The SNPs rs1337185 in COL11A1 and rs162509 in ADAMTS5 are associated with susceptibility to lumbar disc degeneration. The C allele of rs1337185 is risky for patients who are affected by lumbar pathologies such as disc herniation, stenosis and spondylolisthesis. The G allele of rs16250 represents a risk factor for the development of disc herniation. PMID: 28583914
    5. ADAMTS5 is hypermethylated and inhibits cancer cells invasion and migration in colorectal cancer, and correlates with OS and DFS. PMID: 29143120
    6. Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1. PMID: 28306378
    7. MMP-13 may play a role on physiological turnover of cartilage extracellular matrix and that LRP1 is a key modulator of extracellular levels of MMP-13 and its internalization is independent of the levels of ADAMTS-4, -5 and TIMP-3. PMID: 27084377
    8. The IL1B/AP-1/miR-30a/ADAMTS-5 axis regulates cartilage matrix degradation in osteoarthritis. PMID: 27067395
    9. The findings suggest that miR-140 suppresses colorectal cancer progression and metastasis, possibly through downregulating ADAMTS5 and IGFBP5. PMID: 27906093
    10. Results provide direct evidence indicating that Fibulin-2 is a novel substrate of ADAMTS-5 and that this proteolysis could alter the cellular microenvironment affecting the balance between protumor and antitumor effects associated to both Fibulin-2 and the ADAMTSs metalloproteases. PMID: 28099917
    11. Endoplasmic reticulum stress participates in the progress of senescence and apoptosis of osteoarthritic chondrocytes, which manifested in increased expression of ADAMTS5, MMP13, and decreased COL2A1 expression. PMID: 28728848
    12. Single Nucleotide Variants of Candidate Genes in Aggrecan Metabolic Pathway Are Associated with Lumbar Disc Degeneration and Modic Changes PMID: 28081267
    13. RREB1 cooperates with noncoding RNA linc-ADAMTS5 to inhibit ADAMTS5 expression, thereby affecting degeneration of the extracellular matrix (ECM) of the intervertebral disc. PMID: 28341660
    14. Matrilin 2 accumulation associated with relative ADAMTS5 deficiency may contribute to the mechanism underlying calcific aortic valve disease progression. PMID: 28546218
    15. In osteoarthritis (OA) chondrocytes, hydrostatic pressure (HP) restores the expression levels of some miRNAs, downregulates MMP-13, ADAMTS-5, and HDAC-4, and modulates the Wnt/beta-catenin pathway activation. PMID: 28085114
    16. we investigated whether important polymorphisms in the ADAMTS4 and ADAMTS5 genes affect osteoarthritis (OA) susceptibility. ADAMTS4 and ADAMTS5 genotypes were determined using the ABI Prism StepOnePlus Real-Time system. Our findings suggest that the ADAMTS4 (rs4233367 and rs11807350) and ADAMTS5 (rs226794 and rs2830585) variants examined may not contribute to susceptibility to knee OA in the Turkish population. PMID: 27706574
    17. Increased ADAMTS5 levels were observed in placental insufficiency cases. PMID: 27159841
    18. ADAMTS5 protein levels in semen were significantly lower in males with infertility. Sperm count and motility showed a negative correlation with levels of ADAMTS5 protein expression. PMID: 26888488
    19. Overexpression of ADAMTS5 is associated with migration and invasion of non-small cell lung cancer. PMID: 26738863
    20. ADAMTS5 rs2380585 polymorphism contributes to hepatocellular carcinoma susceptibility in a Chinese Han population PMID: 25519309
    21. The findings of our study suggest that hsamiR15a exerts protective effects against osteoarthritis by targeting ADAMTS5 in human chondrocytes. PMID: 26707794
    22. Evaluate ADAMTS-4 and ADAMTS-5 immunohistochemical expression in human TMJ discs from patients affected by internal derangement. PMID: 25477257
    23. The data showed that the expression of ADAMTS5 was reduced in HCC, which was inversely associated with VEGF expression, MVD, and tumor size and associated with poor overall survival of HCC patients. PMID: 25848214
    24. Data suggest that ADAMTS-5 oligomerization is required for full aggrecanase activity in vitro and in situ (as seen in knee joint of mouse model of inflammatory arthritis); thus, blocking oligomerization inhibits ADAMTS-5 activity. PMID: 26668318
    25. These data demonstrate that the antibody is specific to ADAMTS4 and ADAMTS5 and inhibits their aggrecanase activity at molecular and cellular levels. PMID: 26612259
    26. Western blot analysis of endogenous ADAMTS-5 expressed by human chondrocytes showed the presence largely of truncated forms of ADAMTS-5, thus explaining the lack of efficacy of the anti-Sp antibody. PMID: 26303525
    27. We conclude that the upregulation of TNF-alpha and ADAMTS-5, but not ADAMTS-4, may play an important role in degenerative cartilage endplate-induced low back pain. PMID: 24732836
    28. The present study reveals ADAMT-5 expression by mast cells(MCs) and that MC activation regulates the expression of the protease, thus implicating the ADAMT-5 of protease in MC function. PMID: 23154421
    29. The ADAMTS5 ancillary domain and specific chondroitin sulfate chains of versican are required for proteolysis. PMID: 25122765
    30. The restricted expression of ADAMTS-4 and ADAMTS-5 and their increased expression in gestational trophoblastic diseases suggest that these 2 ADAMTS subtypes are associated with human placentation and the development of gestational trophoblastic diseases. PMID: 24786121
    31. psiepsilonRACK induced upregulation of hsa-miR-377 expression, coupled to decreases in ADAMTS5 and cleaved aggrecan PMID: 24312401
    32. No ADAMTS5 rs226794 genotype variant effects are found in association with ATP in two independent populations. PMID: 23491141
    33. This study highlights that the affinity between a ligand and LRP1 dictates the rate of internalization and suggests that LRP1 is a major traffic controller of the two aggrecanases, especially under inflammatory conditions, where the protein levels of ADAMTS-4 increase, but those of ADAMTS-5 do not. PMID: 24474687
    34. Our results suggest that the ADAMTS5 gene polymorphisms may contribute to the susceptibility of osteoarthritis in the Chinese Han population. PMID: 22961118
    35. study indicates the SNPs of ADAMTS-5 may contribute to predisposition of lumbar intervertebral disc degeneration (LDD); an interaction between rs151058 and rs229052 may exist in ADAMTS-5 with LDD; rs162502 might be associated with altered Mean Diffusivity values PMID: 24415654
    36. We have demonstrated that the expression of ADAMTS-1, -4 and -5 is induced during the differentiation of monocytes into macrophages. PMID: 23859810
    37. RelA/p65 is a potent transcriptional activator of ADAMTS5 in chondrocytes during osteoarthritis development. PMID: 23963448
    38. ADAMTS-5/aggrecanase-2 is the main aggrecanase present in laryngeal carcinoma PMID: 23131589
    39. The serine protease tissue plasminogen activator (tPA) and two matrix metalloproteinases, ADAMTS-4 and ADAMTS-5, were identified as Reelin cleaving enzymes. PMID: 23082219
    40. LRP-1 dictates physiological and pathological catabolism of aggrecan in cartilage as a key modulator of the extracellular activity of ADAMTS-5. PMID: 23064555
    41. study showed that ADAMTS-1, -4, -5 and TIMP3 were expressed at differential levels in hepatocellular carcinoma cell lines PMID: 22735305
    42. This is the first report that ADAMTS5 is an anti-angiogenic and anti-tumorigenic protein independent of its proteoglycanase activity. PMID: 22796434
    43. the first evidence implicating ADAMTS-5 in the regulation of proteoglycan turnover and lipoprotein retention in atherosclerosis. PMID: 22493487
    44. Data show that the expression of ADAMTS4, 9, 16 and was up-regulated during chondrogenesis, ADAMTS1 and 5 were down-regulated. PMID: 22562232
    45. a physiological function of ADAMTS5 in dermal fibroblasts is to maintain optimal versican content and PCM volume by continually trimming versican in hyaluronan-versican aggregates. PMID: 21828051
    46. ADAMTS-5 is present in human coronary atherosclerotic plaques. PMID: 21345877
    47. The ADAMTS5 promoter is activated by serum depletion according to promoter reporter assays in HEK 293 cells. PMID: 20494980
    48. Structure analysis with a succinamide inhibitor reveals the flexibility of the ADAMTS-5 active site. PMID: 21370305
    49. four of the six aggrecanases are expressed in immortalized chondrocyte cell-lines and can be upregulated in response to inflammatory cytokines PMID: 20568084
    50. ADAMTS-5 is probably involved in the process of herniated intervertebral disc degeneration, and that IL-1beta-induced expression of ADAMTS-5 is mediated by NO. PMID: 21437951

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
  • 数据库链接：

    HGNC: 221

    OMIM: 605007

    KEGG: hsa:11096

    STRING: 9606.ENSP00000284987

    UniGene: Hs.58324