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  4. Human ADAMTS-like protein 2(ADAMTSL2) ELISA kit

Human ADAMTS-like protein 2(ADAMTSL2) ELISA kit

  • 中文名称：
    人ADAMTS样蛋白2(ADAMTSL2)酶联免疫试剂盒
  • 货号：
    CSB-EL001318HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ADAMTSL2 ELISA Kit was designed for the quantitative measurement of Human ADAMTSL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 别名：
    ADAMTS like 2 ELISA Kit; ADAMTS like protein 2 ELISA Kit; ADAMTS-like protein 2 ELISA Kit; ADAMTSL 2 ELISA Kit; ADAMTSL-2 ELISA Kit; ADAMTSL2 ELISA Kit; ATL2_HUMAN ELISA Kit; FLJ45164 ELISA Kit; KIAA0605 ELISA Kit
  • 缩写：
    ADAMTSL2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTSL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 99
    Range % 94-106
    1:2 Average % 88
    Range % 84-93
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 102
    Range % 98-106
  • 回收率：
    The recovery of human ADAMTSL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 87-96
    EDTA plasma (n=4) 86 81-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 1.854 1.988 1.921 1.812
    100 1.262 1.192 1.227 1.118
    50 0.780 0.733 0.757 0.648
    25 0.487 0.451 0.469 0.360
    12.5 0.312 0.334 0.323 0.214
    6.25 0.231 0.217 0.224 0.115
    3.12 0.169 0.160 0.165 0.056
    0 0.109 0.108 0.109 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

我们正在寻找检测ADAMTSL2的试剂盒，发现了CSB-EL001318HU。该试剂盒能否测量血浆或血清中的内源性ADAMTSL2？该试剂盒使用的免疫原/校准剂是什么？是在某种表达系统中表达的全长重组蛋白么？

A:
本试剂盒的标准品为NS0衍生的重组蛋白。本试剂盒可检测天然样品(血清血浆等)中ADAMTSL2蛋白含量，但每种样品的状态不同，请检查您自己的实际测量值。免疫原:NS0衍生的重组蛋白。

靶点详情

  • 基因功能参考文献：
    1. CpG-specific DNA methylation of ADAMTSL2 and BTN3A2 at rheumatoid arthritis diagnosis can serve as a marker of treatment response. PMID: 28447857
    2. Two compound heterozygous mutations were confirmed in the ADAMTSL2 gene of the patient with geleophysic dysplasia. PMID: 28917829
    3. A novel mutation in ADAMTSL2 (p. Gly421Ser) gene was identified in individuals with Ehlers-Danlos Syndrome. PMID: 26879370
    4. Two novel homozygous missense mutations in the ADAMTSL2 gene underlie geleophysic dysplasia in two consanguineous families from the United Arab Emirates. PMID: 24014090
    5. Molecular screening of ADAMTSL2 gene in 33 patients reveals the genetic heterogeneity of geleophysic dysplasia. PMID: 21415077
    6. These data suggest that ADAMTSL2 mutations may lead to a dysregulation of TGF-beta signaling and may be the underlying mechanism of geleophysic dysplasia. PMID: 18677313

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  • 相关疾病：
    Geleophysic dysplasia 1 (GPHYSD1)
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    HGNC: 14631

    OMIM: 231050

    KEGG: hsa:9719

    STRING: 9606.ENSP00000346478

    UniGene: Hs.522543