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  4. Human Actin-related protein 2/3 complex subunit 2(ARPC2) ELISA kit

Human Actin-related protein 2/3 complex subunit 2(ARPC2) ELISA kit

  • 中文名称：
    人肌动蛋白相关蛋白2/3复合物亚基2(ARPC2)酶联免疫试剂盒
  • 货号：
    CSB-EL002127HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ARPC2 ELISA Kit was designed for the quantitative measurement of Human ARPC2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    Actin related protein 2/3 complex subunit 2 34kDa ELISA Kit; Actin related protein 2/3 complex subunit 2 ELISA Kit; Actin-related protein 2/3 complex subunit 2 ELISA Kit; ARC 34 ELISA Kit; ARC34 ELISA Kit; Arp2/3 complex 34 kDa subunit ELISA Kit; ARP2/3 protein compex subunit 34 ELISA Kit; ARPC 2 ELISA Kit; Arpc2 ELISA Kit; ARPC2_HUMAN ELISA Kit; p34 Arc ELISA Kit; p34-ARC ELISA Kit; p34Arc ELISA Kit; PNAS 139 ELISA Kit; PNAS139 ELISA Kit; PRO 2446 ELISA Kit; PRO2446 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ARPC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %95
    Range %90-105
    1:4Average %99
    Range %92-110
    1:8Average %91
    Range %85-98
  • 回收率：
    The recovery of human ARPC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-97
    EDTA plasma (n=4)9085-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.201 2.122 2.162 2.011
    7501.564 1.476 1.520 1.369
    3751.007 0.953 0.980 0.829
    187.50.683 0.634 0.659 0.508
    940.492 0.453 0.473 0.322
    470.354 0.331 0.343 0.192
    23.50.246 0.239 0.243 0.092
    00.155 0.146 0.151 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the mother actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
  • 基因功能参考文献：
    1. In the association analysis of 110 gastric cancer tissues, ARPC2 showed significant associations with large tumor size, lymph node invasion, and high tumor stage. In addition, ARPC2-positive patients exhibited lower RFS and OS rates compared with ARPC2-negative patients. We thus identify that ARPC2 plays an aneretic role in human gastric cancer and provided a new target for gastric cancer therapy. PMID: 28694563
    2. Downregulation of the ARP2/3 complex signaling pathway, a common final pathway for multiple signaling cascades that regulate the actin cytoskeleton, would compromise the structural stability of spines, leading to their loss. In concert with findings from deletion of the ARP2/3 complex in mice, these findings support the idea that spine deficits in the DLPFC may contribute to subcortical hyperdopaminergia in schizophrenia. PMID: 27523502
    3. Recent reports now demonstrate a novel aspect of the ARP2/3 complex and the nucleating-promoting factors in the maintenance of endothelial barrier function and junction remodeling of established endothelial cell junctions. PMID: 24621569
    4. FOXF1 repressed cell growth and expression of collagen-1 and actin-related protein 2/3 complex, subunit 2. PMID: 25260753
    5. The Arp2/3 complex is recruited to invading Rickettsia parkeri and is required for efficient invasion. PMID: 22188208
    6. Filopodia initiation: focus on the Arp2/3 complex and formins PMID: 21975549
    7. WASH is a bimodular protein and a component of the BLOC-1 complex in which the C terminus is involved in Arp2/3-mediated actin nucleation, whereas the N-terminal portion is required for its regulation and localization in the cells PMID: 20308062
    8. The actin-related protein 2 accumulated in punctate structures that formed an extensivenetwork in a region corresponding to the postition of the Golgi complex. PMID: 15793564
    9. Compared with controls, actin-related protein 2/3 level was markedly increased in brains of intractable epilepsy patients. PMID: 18708039
    10. These findings strongly suggest that defective IL10 function is central to the pathogenesis of the ulcerative colitis subtype of inflammatory bowel disease. PMID: 18836448

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection. Cell junction, synapse, synaptosome. Nucleus.
  • 蛋白家族：
    ARPC2 family
  • 数据库链接：

    HGNC: 705

    OMIM: 604224

    KEGG: hsa:10109

    STRING: 9606.ENSP00000295685

    UniGene: Hs.529303