Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Acylamino-acid-releasing enzyme(APEH) ELISA kit

Human Acylamino-acid-releasing enzyme(APEH) ELISA kit

  • 中文名称：
    人酰基氨基酸释放酶(APEH)酶联免疫试剂盒
  • 货号：
    CSB-EL001899HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human APEH ELISA Kit was designed for the quantitative measurement of Human APEH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    AARE ELISA Kit; ACPH ELISA Kit; ACPH_HUMAN ELISA Kit; Acyl peptide hydrolase ELISA Kit; Acyl-peptide hydrolase ELISA Kit; Acylamino acid releasing enzyme ELISA Kit; Acylamino-acid-releasing enzyme ELISA Kit; Acylaminoacyl peptidase ELISA Kit; Acylaminoacyl-peptidase ELISA Kit; APEH ELISA Kit; APH ELISA Kit; N acylaminoacyl peptide hydrolase ELISA Kit; OPH ELISA Kit; Oxidized protein hydrolase ELISA Kit
  • 缩写：
    APEH
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APEH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 82-87
    1:2 Average % 98
    Range % 94-103
    1:4 Average % 88
    Range % 84-92
    1:8 Average % 108
    Range % 104-112
  • 回收率：
    The recovery of human APEH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 95-103
    EDTA plasma (n=4) 86 86-89
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.049 2.102 2.076 1.960
    1000 1.604 1.586 1.595 1.479
    500 1.080 1.102 1.091 0.975
    250 0.651 0.672 0.662 0.546
    125 0.424 0.402 0.413 0.297
    62.5 0.262 0.271 0.267 0.151
    31.25 0.206 0.199 0.203 0.087
    0 0.117 0.115 0.116 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your ELISA kit CSB-EL001899HU, could you please advise on the following:
1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Does the standard include additive agent such as antiseptic or preservative? If it includes, What is the composition of said additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry!
CSB-EL001899HU
Standard：293 cell-derived recombinant protein.
Preservative is proclin 300(1:3000).
Stop solution is 4N sulfuric acid.
This kit doesn't contain azide, thimerosal, 2-ME, etc.

靶点详情

  • 功能：
    This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
  • 基因功能参考文献：
    1. Although APEH is primarily localized in the cytoplasm, a sub-fraction of this enzyme is sequestered at sites of nuclear damage following UVA irradiation or following oxidative stress. APEH interacts with the amino-terminal domain of XRCC1, and APEH facilitates both single-strand break repair and cell survival following exposure to H2O2. Thus, APEH as a novel proteolytic component of the DNA damage response. PMID: 28866241
    2. APEH polymorphism has significant influence on valproic acid pharmacokinetics in Chinese population PMID: 27406852
    3. Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase S9C family
  • 数据库链接：

    HGNC: 586

    OMIM: 102645

    KEGG: hsa:327

    STRING: 9606.ENSP00000296456

    UniGene: Hs.517969