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Human Adipsin ELISA Kit

  • 中文名称：
    人降脂素酶联免疫试剂盒
  • 货号：
    CSB-E14369h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        CFD是一种补体系统蛋白，其编码基因位于人类基因组上的19号染色体上。CFD是补体系统中的重要成分之一，可以通过被激活后形成C3裂解产物来参与机体的免疫防御。此外，CFD还参与了一些炎症和代谢疾病的发生和发展。
        j9九游会登录入口首页生物所提供的Human Adipsin ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CFD，其灵敏度为0.078 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    Adipsin ELISA Kit; Adipsin/complement factor D ELISA Kit; Adn ELISA Kit; C3 convertase activator ELISA Kit; CFAD_HUMAN ELISA Kit; CFD ELISA Kit; Complement factor D ELISA Kit; complement factor D preproprotein ELISA Kit; D component of complement ELISA Kit; DF ELISA Kit; FactorD ELISA Kit; PFD ELISA Kit; Properdin factor D ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human adipsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:20 Average % 87    
    Range % 82-91    
    1:40 Average % 84    
    Range % 80-88    
    1:80 Average % 104    
    Range % 98-108    
    1:160 Average % 90    
    Range % 85-94    
  • 回收率：
    The recovery of human adipsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 94-103    
    EDTA plasma (n=4) 94 90-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    120 2.591 2.423 2.507 2.402    
    60 1.964 1.890 1.927 1.822    
    30 1.336 1.301 1.319 1.214    
    15 0.842 0.836 0.839 0.734    
    7.5 0.449 0.424 0.437 0.332    
    3.75 0.329 0.339 0.334 0.229    
    1.87 0.249 0.241 0.245 0.140    
    0 0.108 0.102 0.105 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    CFD（Complement factor D）是补体系统中的一种血清蛋白，主要参与剪切促进因子B（Factor B）的激活，从而启动替代途径的活化。最新研究表明，CFD在炎症、自身免疫疾病、感染和肿瘤等多种疾病中都起到了重要作用。例如，CFD可以促进炎症细胞的浸润和激活，导致组织损伤和炎症反应。此外，CFD也参与了肿瘤的生长、侵袭和转移等过程。
     
  • 功能：
    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • 基因功能参考文献：
    1. MASP-3 is the exclusive pro-factor D activator in resting blood: the lectin and the alternative complement pathways are fundamentally linked. PMID: 27535802
    2. disturbance of reciprocal relationships between adipsin and leptin in obesity is associated with the development of insulin resistance PMID: 28864895
    3. TZDs (pioglitazone, rosiglitazone, and ciglitazone) slow tumor cell growth in vitro and in vivo with decreases in cell proliferation and increases in PPARg and adipsin PMID: 28219679
    4. The strong association of PLIN1, CFD and ADIPOQ genes with adipogenesis prompted s to study the influence the bone health status as evaluated by quantitative ultrasound (QUS) bone densitometer in a North Indian cohort. Overall, ADIPOQ (rs1501299 and rs3774261) and combined cluster of PLIN1 rs2304796 and rs2304795) and CFD (rs1683563) demonstrated correlation. PMID: 28189761
    5. our findings set up a novel mechanism for FABP4, adipsin and adiponectin through gut microbiota mediating expression in gut Paneth cells. PMID: 26687459
    6. Overall adipsin levels among male asbestos industry workers were significantly higher than among control subjects. PMID: 25840635
    7. Data indicate that a urinary protein, adipsin, was significantly increased in patients with preeclampsia. PMID: 24958499
    8. Study demonstrates that T2DM patients with beta cell failure are deficient in adipsin. PMID: 24995977
    9. Complement factor D (FD) is activated by its substrate through interactions outside the active site which lock the unbound native state into an ordered inactive conformation via the self-inhibitory loop in FD. PMID: 24598742
    10. We demonstrate novel secretion of adipsin and ASP by placental Hofbauer cells. PMID: 23956345
    11. Increased activation of the alternative complement pathway in vitreous was controlled by disease stage and genetic variation in the complement pathway, supporting a role for complement activation in macular degeneration disease pathogenesis. PMID: 22930722
    12. Anti factor D Fab fragment inhibits FD proteolytic function by interfering with macromolecular substrate access rather than by inhibiting FD catalysis. PMID: 22362762
    13. CFD regulates activation of the alternative complement pathway, which is implicated in age related macular degeneration pathogenesis. PMID: 22003108
    14. CFD may not play a major role in the genetic susceptibility to age-related macular degeneration. PMID: 21139680
    15. crystal structure of C3bB at 4 A and complex with factor D at 3.5 A; data show how factor B binding to C3b forms open "activation" state of C3bB; Factor D binds open conformation of factor B through a site distant from the catalytic center PMID: 21205667
    16. As adiponectin and adipsin levels in CSF did not correlate with their levels in plasma, it seems that there could be a secondary intrathecal synthesis of these adipocytokines in multiple sclerosis PMID: 19538214
    17. increased serum levels in patients with seasonal allergic rhinitis PMID: 19699327

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  • 相关疾病：
    Complement factor D deficiency (CFDD)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    HGNC: 2771

    OMIM: 134350

    KEGG: hsa:1675

    STRING: 9606.ENSP00000332139

    UniGene: Hs.155597