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  4. Human Agouti Related Protein,AGRP ELISA Kit

Human Agouti Related Protein,AGRP ELISA Kit

  • 中文名称：
    人Agouti相关蛋白,AGRP酶联免疫试剂盒
  • 货号：
    CSB-E09299h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        Agouti 相关蛋白 (AGRP) 是一种肽类激素，在调节能量平衡和新陈代谢方面起着重要作用。它由下丘脑中的神经元产生，其表达受瘦素调节。 AGRP 已被证明参与食物摄入、体重、脂肪储存和葡萄糖代谢的调节。它还与肥胖和其他代谢紊乱（如 2 型糖尿病）的发病机制有关。除了其生理作用外，AGRP 还被确定为这些疾病治疗干预的潜在靶点。
        j9九游会登录入口首页生物所提供的Human Agouti Related Protein,AGRP ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、细胞培养上清、尿液样本中的AGRP，其灵敏度为0.002 pg/mL，检测范围为31.25 pg/mL-2000 pg/mL。
     
  • 别名：
    Agouti related neuropeptide ELISA Kit; Agouti Related Protein Homolog ELISA Kit; Agouti-related protein ELISA Kit; Agrp ELISA Kit; AGRP_HUMAN ELISA Kit; AGRT ELISA Kit; ART ELISA Kit; ASIP2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, urine
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    0.002 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

     

    Three samples of known concentration were tested twenty times on one plate to assess.

     

    Inter-assay Precision (Precision between assays): CV%<10%

     

    Three samples of known concentration were tested in twenty assays to assess.

      

  • 线性度：

     To assess the linearity of the assay, samples were spiked with high concentrations of human AGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 标准曲线：

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
        Agouti相关蛋白（Agouti-related protein，AGRP）是一种由下丘脑神经元分泌的神经肽，其在食欲和能量平衡调节中发挥重要作用。最新的研究进展如下：
        AGRP在肥胖和糖尿病中的作用：AGRP已被证明是食欲和体重增加的主要因素之一。最新的研究表明，AGRP与肥胖和糖尿病密切相关。研究人员发现，AGRP与胰岛素抵抗和胰岛素分泌异常有关，因此成为研究和治疗糖尿病的潜在靶点。
        AGRP与神经系统疾病的关系：AGRP也与一些神经系统疾病有关，如帕金森病、亚急性硬化性脑炎等。最新的研究表明，AGRP通过对神经元和胶质细胞的作用，可能参与调节神经炎症反应和神经退行性疾病的进程。
        AGRP与生殖和性腺疾病的关系：AGRP在性腺功能和生殖过程中也发挥一定的作用。最新的研究表明，AGRP可能参与调节青春期和生殖周期的调节，与一些性腺疾病如多囊卵巢综合症等有关。
        AGRP与睡眠障碍的关系：AGRP也可能与睡眠障碍有关。最新的研究表明，AGRP能够通过调节脑内的睡眠调节中枢，影响睡眠质量和睡眠时长。
  • 功能：
    Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.
  • 基因功能参考文献：
    1. AgRP is not associated with changes in hunger or satiety, and can change without corresponding changes in leptin. This suggests that AgRP may not be involved in the episodic control of appetite and the release of AgRP may involve signals other than leptin. PMID: 27476955
    2. data provide compelling evidence that AgRP is a heparan sulfate-binding protein and localizes critical regions in the AgRP structure required for this interaction. PMID: 28264929
    3. The aim of this survey is to evaluate the association of genetic variants of melanocortin-4-receptor (MC4R), pro-opiomelanocortin (POMC), apolipoprotein E (APOE) and agouti-related protein (AGRP) with obesity in the North Indian population. PMID: 26226973
    4. CSF AgRP was not different in lean vs. overweight/obese subjects; however, plasma AgRP was higher in lean subjects. PMID: 26152765
    5. human agouti-related protein has a role in enhancing expression and stability of human melanocortin-4 receptor PMID: 25446108
    6. Variations in genes AGRP, CPE, GHRL, GLP1R, HTR2A, NPY1R, NPY5R, SOCS3 and STAT3 showed modest associations with BMI in European Americans. PMID: 23900445
    7. Elevations in AgRP also favor the diagnosis of ectopic ACTH syndrome, suggesting AgRP should be further evaluated as a potential neuroendocrine tumor marker. PMID: 25013995
    8. AGRP SNP (rs1338993) was not associated with antipsychotic-induced weight gain in schizophrenia patients. PMID: 24564533
    9. Data suggest that prolonged steady-state exercise/physical activity at moderate intensity elicits substantial increases in blood lactate and cortisol concentrations without a significant change in AgRP; study was pilot project involving men. PMID: 22477065
    10. Despite peripheral hyperleptinemia, positive energy balance is achieved during pregnancy by a relative decrease in central leptin concentrations and resistance to leptin's effects on target neuropeptides that regulate energy balance. PMID: 23118421
    11. AgRP and NPY are correlated with body weight changes, rather than the presence of type 2 diabetes, whereas changes in alphaMSH immunoreactivity are related to the presence of type 2 diabetes, indicating separate hypothalamic mechanisms. PMID: 22492775
    12. Cognitive flexibility plays an important role in anorexia nervosa and may be modulated by abnormal levels of the appetite-regulating peptide AGRP. PMID: 21531082
    13. This review defines how peripheral signals (particularly leptin, insulin and glucose) converge on a molecular level in proopiomelanocortin (POMC)- and agouti-related protein (AgRP)-expressing arcuate nucleus neurons to control energy homeostasis. PMID: 19770186
    14. Only the patients with nonalcoholic fatty liver presented a positive correlation between the saturated fatty acids intake and the orexigenic neuropeptides NPY and agouti related protein, and carbohydrate with NPY PMID: 20164781
    15. AgRP may provide an important signal in the immune environment and the lymphocyte may be considered as an extra-hypothalamic source of plasma AgRP following exercise stress PMID: 20404050
    16. Agouti-related protein is neatively correlated with nonalcolic fatty liver disease in obese adolescents. PMID: 19942238
    17. A polymorphism, 199G>A, resulted in substitution of the amino acid at position 67 from alanine to threonine. PMID: 11602360
    18. NMR structural studies show that the loop in the first 16 residues of the C-terminal domain of AGRP confers distinct selectivity for melanocortin receptors MC3R and MC4R. PMID: 11747427
    19. the c.199G-->A polymorphism in hAGRP could play a role in the development of human obesity in an age-dependent fashion PMID: 12213871
    20. Ala67Thr polymorphism in the Agouti-related peptide gene is associated with inherited leanness in humans PMID: 15054840
    21. Two single nucleotide polymorphisms in the AGRP promotor showed opposite effects on promotor activity. PMID: 15121772
    22. Serum levels could serve as useful peripheral markers of changes in energy homeostasis. PMID: 15546902
    23. the C-terminus of AgRP can participate in the insulin secretion in pancreatic beta cells, through the modulation of calcium release PMID: 15978665
    24. Single-nucleotide polymorphisms found in anorexia nervosa. PMID: 16314751
    25. AgRP may have a novel inhibitory paracrine role in the human adrenal gland PMID: 16320160
    26. analysis of molecular basis of melanocortin-4 receptor for AGRP inverse agonism PMID: 16820227
    27. Plasma Agouti-related protein (AGRP)elevation may be related to energy homeostasis disturbance in anorexia nervosa, and in addition to leptin, peripheral AGRP levels could be used as a nutritional marker in AN patients. PMID: 16904835
    28. in addition to its inverse agonistic activities, Agrp exhibits agonistic properties on the endocytosis pathway of melanocortin-3 and -4 receptors PMID: 17041250
    29. a rare mutation, +79G>A, was identified in the AGRP minimal promoter; the +79G>A mutation could predispose to body weight gain PMID: 17180153
    30. two adjacent enhancers inside the first intron of the neighboring (1.4 kb downstream) ATPase gene (ATP6V0D1) modulate the human AgRP promoter with profound spatiotemporal variation PMID: 19285986
    31. This study presents an association of rare allele of AGRP polymorphism in heterozygous state with increased body mass index PMID: 19602223
    32. The high expressing C/C genotype of AGRP was significantly associated with high BMI and type 2 diabetes in Africans. PMID: 11554767

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  • 相关疾病：
    Obesity (OBESITY)
  • 亚细胞定位：
    Secreted. Golgi apparatus lumen.
  • 组织特异性：
    Expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
  • 数据库链接：

    HGNC: 330

    OMIM: 601665

    KEGG: hsa:181

    STRING: 9606.ENSP00000290953

    UniGene: Hs.104633