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Human Alpha-N-acetylgalactosaminidase(NAGA) ELISA kit

  • 中文名称：
    人α-N-乙酰氨基半乳糖苷酶(NAGA)酶联免疫试剂盒
  • 货号：
    CSB-EL015414HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human NAGA ELISA Kit was designed for the quantitative measurement of Human NAGA protein in serum, plasma, urine, saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    NAGA ELISA Kit; Alpha-N-acetylgalactosaminidase ELISA Kit; EC 3.2.1.49 ELISA Kit; Alpha-galactosidase B ELISA Kit
  • 缩写：
    NAGA
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, saliva, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NAGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 97  
    Range % 92-101  
    1:4 Average % 86  
    Range % 82-90  
    1:8 Average % 105  
    Range % 100-109  
  • 回收率：
    The recovery of human NAGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.246 2.152 2.199 1.980  
    20 1.892 1.815 1.854 1.635  
    10 1.465 1.504 1.485 1.266  
    5 1.127 1.179 1.153 0.934  
    2.5 0.874 0.836 0.855 0.636  
    1.25 0.541 0.568 0.555 0.336  
    0.625 0.362 0.349 0.356 0.137  
    0 0.221 0.217 0.219    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.
  • 基因功能参考文献：
    1. results demonstrate the utility of eQTL mapping in the identification of novel asthma genes and provide evidence for the importance of FADS2, NAGA, and F13A1 in the pathogenesis of asthma. PMID: 24934276
    2. the active sites of human lysosomal enzymes alpha-galactosidase and alpha-N-acetylgalactosaminidase have interconvertible specificites PMID: 20444686
    3. Tumor cell alpha-N-acetylgalactosaminidase activity and its involvement in GcMAF-related macrophage activation. PMID: 12062184
    4. Structural analysis revealed biochemical and phenotypic differences in these Kanzaki patients with the R329Q and R329W mutation. PMID: 15619430
    5. NAGA mutation p.D217N (c.649G>A) in exon 6 and mutation p.E325K (c.973G>A) in exon 8 may have roles in alpha-N-acetylgalactosaminidase deficiency with cardiomyopathy PMID: 17171432
    6. Specific enzymatic activity of serum alpha-N-acetylgalactosaminidase was significantly increased in stage III melanoma patients, but not in early stages. PMID: 19394758
    7. The active site of human alpha-NAGAL has anomeric selectivity for its catalytic product and the structure reveals a novel active-site rearrangement upon hexose ligand binding. PMID: 19683538
    8. Use of a modified NAGA in the development of enzyme replacement therapy for Fabry disease is reported. PMID: 19853240

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  • 相关疾病：
    Schindler disease (SCHIND); Kanzaki disease (KANZD)
  • 亚细胞定位：
    Lysosome.
  • 蛋白家族：
    Glycosyl hydrolase 27 family
  • 数据库链接：

    HGNC: 7631

    OMIM: 104170

    KEGG: hsa:4668

    STRING: 9606.ENSP00000379680

    UniGene: Hs.75372