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Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit

  • 中文名称:
    人血管紧张素Ⅱ受体2抗体(AT2R-Ab)酶联免疫试剂盒
  • 货号:
    CSB-E11255h
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human AT2R Ab ELISA Kit was designed for the quantitative measurement of Human AT2R Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 U/mL-20 U/mL and the sensitivity is 0.078 U/mL.

  • 别名:
    angiotensin 2 receptor 2 antibody, AT2R-Ab
  • 缩写:
    AT2R Ab
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    0.312 U/mL-20 U/mL
  • 灵敏度:
    0.078 U/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human AT2R-Ab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 82-105    
    1:2 Average % 94    
    Range % 84-100    
    1:4 Average % 98    
    Range % 92-101    
    1:8 Average % 94    
    Range % 82-105    
  • 回收率:
    The recovery of human AT2R-Ab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 83-105    
    EDTA plasma (n=4) 95 85-103    
                   
                   
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average Corrected    
    20 2.635 2.659 2.647 2.501    
    10 1.963 1.927 1.945 1.799    
    5 1.296 1.287 1.292 1.146    
    2.5 0.771 0.748 0.760 0.614    
    1.25 0.468 0.492 0.480 0.334    
    0.625 0.346 0.351 0.349 0.203    
    0.312 0.255 0.246 0.251 0.105    
    0 0.147 0.145 0.146      
  • 数据处理:
  • 货期:
    3-5 working days

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