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  4. Human Ankyrin repeat domain-containing protein 26(ANKRD26) ELISA kit

Human Ankyrin repeat domain-containing protein 26(ANKRD26) ELISA kit

  • 中文名称：
    人锚蛋白重复结构域蛋白26(ANKRD26)酶联免疫试剂盒
  • 货号：
    CSB-EL001763HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ANKRD26 ELISA Kit was designed for the quantitative measurement of Human ANKRD26 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    Ankrd26 ELISA Kit; Ankyrin repeat domain 26 ELISA Kit; Ankyrin repeat domain-containing protein 26 ELISA Kit; Ankyrin repeat domaincontaining protein 26 ELISA Kit; ANR26_HUMAN ELISA Kit; bA145E8.1 ELISA Kit; KIAA1074 ELISA Kit
  • 缩写：
    ANKRD26
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ANKRD26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 87-93
    1:2 Average % 102
    Range % 98-106
    1:4 Average % 85
    Range % 80-91
    1:8 Average % 104
    Range % 101-108
  • 回收率：
    The recovery of human ANKRD26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 95-103
    EDTA plasma (n=4) 86 83-89
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.126 2.201 2.164 2.039
    1000 1.671 1.658 1.665 1.540
    500 1.135 1.204 1.170 1.045
    250 0.696 0.685 0.691 0.566
    125 0.464 0.471 0.468 0.343
    62.5 0.318 0.320 0.319 0.194
    31.25 0.241 0.249 0.245 0.120
    0 0.126 0.124 0.125 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Acts as a regulator of adipogenesis. Involved in the regulation of the feeding behavior.
  • 基因功能参考文献：
    1. in a cohort of patients with suspected familial thrombocytopenia, the c.-140C>G mutation seems to be the most frequent ANKRD26 mutation. PMID: 28277066
    2. Two cases with mutant ANKRD26 highlight that patients with thrombocytopenia 2 are at risk of being misdiagnosed with myelodysplastic syndrome and receiving undue myelosuppressive treatments. Because dysmegakaryopoiesis is a feature also of other forms of inherited thrombocytopenia, a genetic disorder must always be considered when a patient presents with isolated thrombocytopenia and dysmegakaryopoiesis. PMID: 28976612
    3. investigation of one patient with the c.3G>A showed that mutation was associated with strong ANKRD26 overexpression in vivo, which is the proposed mechanism for predisposition to AML in THC2 patients PMID: 28100250
    4. The findings of lifelong thrombocytopenia with mild/absent bleeding, family history of thrombocytopenia with normal platelet size and myeloid neoplasms should raise the suspicion of ANKRD26 mutated thrombocytopenia. PMID: 27123948
    5. Molecular analysis identified a mutation located in the promoter of the ankyrin repeat domain 26 (ANKRD26) gene, c.-127A>T in normocytic thrombocytopenia. PMID: 27108925
    6. thrombocytopenia with 5'UTR ANKRD26 gene mutation must be considered in case of a constitutional isolated thrombocytopenia, with a low bleeding tendency, associated with autosomal dominant transmission and normal platelet volume. PMID: 25902755
    7. WASP, RUNX1, and ANKRD26 genes are important for normal TPO signaling and the network underlying thrombopoiesis. PMID: 26175287
    8. The study supports the association of ANKRD26 mutations with thrombocytopenia 2 and a predisposition to myeloid malignancies. PMID: 24628296
    9. ANKRD26 regulatory region mutations induce MAPK hyperactivation in familial thrombocytopenia PMID: 24430186
    10. the missense mutations may paly a role in the pathogenesis of Autosomal-dominant nonsyndromic thrombocytopenia-2 PMID: 23869080
    11. Studies indicate that ANKRD26-RT is an insidious form of inherited thrombocytopenias that exposes patients to a low risk of bleeding but predisposes them to hematologic myeloid malignancies. PMID: 24030261
    12. Ubiquitin/proteasome-rich particulate cytoplasmic structures are a characteristic feature of ANKRD26-related thrombocytopenia platelets and megakaryocytes. PMID: 23223974
    13. The ANKRD26-related thrombocytopenia has to be taken into consideration in the differential diagnosis of isolated thrombocytopenias. PMID: 21467542
    14. mutations in the 5' UTR of ANKRD26 are implicated in thrombocytopenia 2. PMID: 21211618

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  • 相关疾病：
    Thrombocytopenia 2 (THC2)
  • 数据库链接：

    HGNC: 29186

    OMIM: 188000

    KEGG: hsa:22852

    STRING: 9606.ENSP00000365255

    UniGene: Hs.361041