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  4. Human Annexin Ⅴ,ANX-Ⅴ ELISA Kit

Human Annexin Ⅴ,ANX-Ⅴ ELISA Kit

  • 中文名称：
    人膜联蛋白Ⅴ(ANX-Ⅴ)酶联免疫试剂盒
  • 货号：
    CSB-E04882h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The ELISA Kit is designed to measure human ANXA5 levels in samples, including serum, plasma, cell culture supernates, or cell lysates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ANXA5 in samples. The color intensity is measured at 450 nm via a microplate reader.

    ANXA5 is known to possess anti-inflammatory and anti-apoptotic properties. It also has a role in cytotoxicity. Due to its preferential phosphatidylserine (PS) binding property, ANXA5 has been used as a marker for the detection of apoptosis-undergoing cells. It regulates cell proliferation, signal transduction, and coagulation. In addition, it has been shown that the binding of ANXA5 to PS+ apoptotic cells can regulate the PS-mediated immunosuppressive clearance, thus enhancing the immunogenicity of apoptotic cells, including irradiated, apoptotic-tumor cells. Also, overexpression of ANXA5 has been detected in many cancers, including renal cell cancer, hepatocellular carcinoma, colorectal cancer, and breast cancer.

  • 别名：
    Anchorin CII ELISA Kit; Annexin 5 ELISA Kit; Annexin A5 ELISA Kit; Annexin V ELISA Kit; Annexin-5 ELISA Kit; Annexin5 ELISA Kit; AnnexinA5 ELISA Kit; AnnexinV ELISA Kit; ANX 5 ELISA Kit; ANX A5 ELISA Kit; ANX5 ELISA Kit; ANXA5 ELISA Kit; ANXA5_HUMAN ELISA Kit; Calphobindin I ELISA Kit; CBP-I ELISA Kit; Endonexin II ELISA Kit; ENX 2 ELISA Kit; ENX2 ELISA Kit; Lipocortin V ELISA Kit; PAP I ELISA Kit; PAP-I ELISA Kit; Placental anticoagulant protein 4 ELISA Kit; Placental anticoagulant protein I ELISA Kit; PLACENTAL PROTEIN 4 ELISA Kit; PP 4 ELISA Kit; Pp4 ELISA Kit; RPRGL3 ELISA Kit; Thromboplastin inhibitor ELISA Kit; VAC-alpha ELISA Kit; Vascular anticoagulant-alpha ELISA Kit
  • 缩写：
    ANXA5
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-V in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 107    
    Range % 96-112    
    1:2 Average % 101    
    Range % 91-107    
    1:4 Average % 97    
    Range % 86-103    
    1:8 Average % 103    
    Range % 95-107    
  • 回收率：
    The recovery of human ANX-V spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 100 90-106    
    EDTA plasma (n=4) 102 95-107    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    20 2.722 2.635 2.679 2.556    
    10 2.081 1.963 2.022 1.899    
    5 1.455 1.401 1.428 1.305    
    2.5 0.948 0.917 0.933 0.810    
    1.25 0.623 0.608 0.616 0.493    
    0.625 0.339 0.326 0.333 0.210    
    0.312 0.231 0.229 0.230 0.107    
    0 0.125 0.121 0.123 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 基因功能参考文献：
    1. AnxA5 2D-network mediates E-cadherin mobility in the plasmalemma that triggers human trophoblasts aggregation and thereby cell fusion. PMID: 28176826
    2. Caspase-3 and -8 and annexin V may serve as diagnostic markers in Ovarian cancer , also explained that the decrement in control of the S phase in the cell cycle may considered one of the significant factors in the development of ovarian tumors PMID: 30197345
    3. When ANXA5 expression increased, cell proliferation was inhibited by regulating the expression of bcl-2 and bax while cell metastasis was suppressed by regulating E-cadherin and MMP-9 expression. PMID: 30010106
    4. Maternal carriers of the ANXA5 M2 haplotype has greater risk of developing placenta-mediated pregnancy complications, such as preeclampsia and intrauterine growth restriction. PMID: 29497952
    5. The domain IV-truncated form of anxA5 is impaired in binding phosphatidylserine liposome and apoptotic cells, and anticoagulation activity. PMID: 29257055
    6. increase in VEGF and Apelin, and decrease in Annexin A5 supports roles of hemo-dynamic alterations in fetoplacental circulation and structural alterations in uteroplacental bed occurring in preeclampsia. PMID: 29208172
    7. In patients with colon cancer, annexin A5 expression in cancer tissues is related to lymph node metastasis and tumor grade. Serum level of annexin A5 is related to annexin A5 expression in cancer tissues. PMID: 29093625
    8. annexin A5 anticoagulant has a role in adverse outcomes in antiphospholipid antibody positive patients with thrombosis or pregnancy complications PMID: 28393472
    9. There was no significant difference in circulating ANXA5 levels between subjects of the non-pregnant, RPL and parous groups (median, 0.25 ng/ml vs. 0.21 ng/ml vs. 0.01 ng/ml, p = 0.14) (Fig. 1A ). Similarly, no significant differences in circulating ANXA5 levels were observed across these three clinical subgroups among M2-carriers or non-carriers, respectively. PMID: 28605660
    10. Serum annexin A5 level was not altered in pre-eclampsia. PMID: 28501283
    11. Annexin A5 was proposed to use in detection of apoptosis. associated with the Breast Cancer. Upregulation of the AA5 protein is detected in Pleomorphic Adenoma of the Parotid Gland. PMID: 28497265
    12. Physiological function of ANXA5 as an embryonic anticoagulant that appears deficient in contiguous specter of thrombophilia-related pregnancy complications culminating more frequently in miscarriage in a maternal M2 carrier background. PMID: 28900802
    13. This study confirmed the proposed role of M2/ANXA5 as embryonic, genetically associated thrombophilia predisposition factor for early recurrent pregnancy loss among ethnic Malay of Malaysia. PMID: 28108842
    14. Circulating anxA5 levels are associated with carotid intima-media thickness but not coronary plaque composition in high-risk patients with diabetes mellitus. PMID: 28592134
    15. Results showed that ANXA5 rs1050606 was significantly associated with left ventricular hypertrophy in Chinese endogenous hypertension patients. PMID: 29095261
    16. Moreover, mechanism study exhibited that Annexin A5 could activate PI3K/Akt/mTOR signaling pathway, promote epithelial-mesenchymal transition (EMT) and the expression of MMP2 and MMP9. Annexin A5 may be a potential prognostic biomarker in RCC and promotes proliferation, migration and invasion of RCC cells via activating PI3K/Akt/mTOR signaling pathway and regulating EMT process and MMP expression. PMID: 28393205
    17. human myotubes rendered deficient for Annexin-A5 (AnxA5) suffer from a severe defect in membrane resealing. PMID: 27286750
    18. This study functionally characterized allelic variants in the ANXA5 promoter, both alone and in combinations, and the results suggest that combinations of several individual variants contribute to modulate the ANXA5 transcriptional activity, most likely through binding of nuclear factors. PMID: 27318245
    19. Chronically increased alveolar annexin V levels, as reflected in increased idiopathic pulmonary fibrosis (IPF) BALF levels, may contribute to the progression of IPF by inducing the release of pro-fibrotic mediators. PMID: 26160872
    20. lower exhaled breath condensate annexin A5 levels in children with exercise-induced asthma compared to non-exercised-induced one PMID: 25796304
    21. The M2 haplotype is a risk factor for early spontaneous abortions, before the 12th week of gestation, and confers about the same relative risk to carriers of both sexes. PMID: 26371709
    22. annexin A5 promoter haplotype M2 may not have a role in recurrent pregnancy loss in Estonia or Denmark PMID: 26135579
    23. Annexin V is fused to an enzyme, creating a fusion protein that converts nontoxic drug precursors, prodrugs, into anticancer compounds while bound to the tumor, therefore mitigating the risk of side effects. PMID: 25899647
    24. Neither the M1/M2 haplotypes nor the tag SNPs in ANXA5 were convincingly associated with pregnancy related venous thromboembolism. PMID: 25495894
    25. Anx5 could protect cardiomyocytes adherens junctions and improve myocardial contractile function via regulation of p120 and anti-inflammation in LPS-induced endotoxemia. PMID: 25799159
    26. The associated male partner risk confirms the proposed role of M2/ANXA5 as a genetic trait impeding embryonic anticoagulation. PMID: 25682309
    27. AnxA5 promotes membrane resealing of injured human trophoblasts. PMID: 25595530
    28. ANXA5 haplotypes/plasma ANXA5 levels were not associated with carotid IMT progression or cardiovascular disease risk in familial hypercholesterolemia patients. PMID: 25525746
    29. The prognostic value of AnV and T cell apoptosis was evaluated in children with nephrotic syndrome in this review. PMID: 25661914
    30. study found neither individual SNPs nor any of the four haplotypes within the ANXA5 gene upstream region were associated with deep venous thrombosis in the Dutch population PMID: 25382354
    31. Lower incidence of M2/ANXA5 carriage in recurrent pregnancy loss patients with elevated lipoprotein(a) levels. PMID: 24335248
    32. Polycystic ovary syndrome subjects showed increased annexin V positive microparticle concentrations compared with healthy volunteers. PMID: 25336711
    33. Annexin A5 inhibits DLBCL cell invasion, MMP-9 expression/activity, and chemoresistance to CHOP through a PI3K-dependent mechanism. PMID: 25323007
    34. Data show involvement of ANXA5 and ILKAP in susceptibility to malignant melanoma PMID: 24743186
    35. annexin A5 promotes GBM cell invasion, MMP-2 expression/activity, and chemoresistance to temozolomide through a PI3K-dependent mechanism. PMID: 25245332
    36. Modified forms of LDL activate human T cells through dendritic cells. Annexin A5 inhibits these effects and promotes induction of regulatory T cells. PMID: 25395618
    37. The annexin 5 in serums of pregnant women and patients with particular types of cancer PMID: 25080795
    38. This review describes the structure, function and genetic expression of ANXA5, as well as its possible implication in recurrent pregnancy loss. PMID: 24152411
    39. We could not prove any association of ANX5 antibodies and systemic lupus erythematosis. PMID: 24872142
    40. genetic association studies in population in Japan: Data suggest that hypomorphic alleles in ANXA5 promoter in placenta (that is, fetal alleles), but not in maternal blood, contributes to onset of pre-eclampsia. PMID: 24140079
    41. This study demonstrated the significant changes in AnxV levels and its function in type 1 diabetic patients. PMID: 24423325
    42. Annexin A5 binds in a cooperative manner to to phosphatidylserine on apoptotic cell membranes. Shrunken apoptotic cells showed the highest Hill coefficient values. PMID: 24304966
    43. frequency of annexin A5 (-1C/T) polymorphism was higher in systemic lupus erythematosus related groups but did not correlate with recurrent pregnancy loss (RPL) or annexin A5 level; anti-annexin A5 IgM level was higher among antiphospholipid syndrome patients and was associated with RPL PMID: 24600987
    44. Annexin-V modified substrate was constructed via layer-by-layer (LBL) method for specific capture of early stage apoptotic Jurkat cells. PMID: 24021657
    45. annexin A5 may play a crucial role in cisplatin-induced toxicity by mediating the mitochondrial apoptotic pathway via the induction and oligomerization of VDAC. PMID: 24318879
    46. Suggest that annezin A5 IgG plays a significant role in producing a hypercoagulable state in primary and secondary antiphospholipid syndrome. PMID: 23981755
    47. Variations within the ANXA5 gene upstream region were confirmed to be risk factors of recurrent pregnancy loss. PMID: 23850300
    48. rs11575945 polymorphism of the annexin-A5 gene is associated with schizophrenia, and its minor allele is responsible for higher levels of the annexin-A5 protein in the blood and represent one of the risk factors for this disease PMID: 24466757
    49. ANXA5 M2 haplotpye appears as an recurrent pregnany loss risk factor in male and female carriers with most distinguishable effects between the 10th and 15th week of gestation. PMID: 23899942
    50. It appears that the haplotype M2/ANXA5 is not associated with the presence of anti-trophoblast antibodies. PMID: 23529182

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  • 相关疾病：
    Pregnancy loss, recurrent, 3 (RPRGL3)
  • 蛋白家族：
    Annexin family
  • 数据库链接：

    HGNC: 543

    OMIM: 131230

    KEGG: hsa:308

    STRING: 9606.ENSP00000296511

    UniGene: Hs.480653