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  4. Human Apolipoprotein F(APOF) ELISA kit

Human Apolipoprotein F(APOF) ELISA kit

  • 中文名称：
    人载脂蛋白F(APOF)酶联免疫试剂盒
  • 货号：
    CSB-EL001938HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human APOF ELISA Kit was designed for the quantitative measurement of Human APOF protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 9.38 ng/mL-600 ng/mL and the sensitivity is 2.34 ng/mL.

  • 别名：
    Apo-F ELISA Kit; Apof ELISA Kit; APOF_HUMAN ELISA Kit; Apolipoprotein F ELISA Kit; DKFZp781G18150 ELISA Kit; Lipid transfer inhibitor protein ELISA Kit; LTIP ELISA Kit; MGC22520 ELISA Kit
  • 缩写：
    APOF
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    9.38 ng/mL-600 ng/mL
  • 灵敏度：
    2.34 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APOF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 92
    Range % 86-96
    1:400 Average % 95
    Range % 91-99
    1:800 Average % 88
    Range % 84-94
    1:1600 Average % 87
    Range % 84-93
  • 回收率：
    The recovery of human APOF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-98
    EDTA plasma (n=4) 95 90-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average
    600 0.088 0.090 0.089
    300 0.113 0.119 0.116
    150 0.161 0.172 0.167
    75 0.243 0.254 0.249
    37.5 0.388 0.395 0.392
    18.75 0.680 0.698 0.689
    9.38 0.941 1.001 0.971
    0 1.975 2.075 2.025
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
  • 基因功能参考文献：
    1. Direct interactions between C/EBPalpha and ETS-1 were important for high liver-specific expression of ApoF. PMID: 25726912
    2. this study has identified apoF as likely another target gene of FXR. PMID: 24211198
    3. LTIP translocation is dependent on LDL lipid composition PMID: 21937674
    4. LTIP tailors CETP-mediated remodeling of HDL3 and HDL2 particles in subclass-specific ways, strongly implicating it as a regulator of HDL metabolism PMID: 12907677
    5. we have confirmed the negative association between plasma triglyceride levels and Apolipoprotein F previously suggested by a small data set, but now we demonstrate that this effect is seen only in males. PMID: 17901467
    6. lipoprotein composition may influence the status of lipid transfer inhibitor protein activity PMID: 18369235

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein F family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    HGNC: 615

    OMIM: 107760

    KEGG: hsa:319

    STRING: 9606.ENSP00000381250

    UniGene: Hs.534302