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  4. Human Apolipoprotein O(APOO) ELISA kit

Human Apolipoprotein O(APOO) ELISA kit

  • 中文名称：
    人载脂蛋白O(APOO)酶联免疫试剂盒
  • 货号：
    CSB-EL001948HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human APOO ELISA Kit was designed for the quantitative measurement of Human APOO protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.08 ng/mL.

  • 别名：
    APOO; FAM121B; MIC23; MIC26; My025; UNQ1866/PRO4302; MICOS complex subunit MIC26; Apolipoprotein O; MICOS complex subunit MIC23; Protein FAM121B
  • 缩写：
    APOO
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.08 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APOO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 102    
    Range % 97-106    
    1:200 Average % 86    
    Range % 83-91    
    1:400 Average % 108    
    Range % 104-112    
    1:800 Average % 85    
    Range % 81-90    
  • 回收率：
    The recovery of human APOO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 97-106    
    EDTA plasma (n=4) 89 85-96    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    20 0.122 0.126 0.124      
    10 0.207 0.215 0.211      
    5 0.326 0.331 0.329      
    2.5 0.553 0.549 0.551      
    1.25 0.846 0.854 0.850      
    0.625 1.197 1.200 1.199      
    0.312 1.586 1.598 1.592      
    0 2.387 2.400 2.394      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function. Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL.
  • 基因功能参考文献：
    1. The human apolipoprotein MIC26 is a bona fide subunit of the MICOS complex. PMID: 25764979
    2. It is likely that apoO participates in fatty acid metabolism and the inflammatory response in HepG2 cells. PMID: 24341743
    3. APOO represents a link between impaired mitochondrial function and cardiomyopathy onset, and targeting APOO-dependent metabolic remodeling has potential as a strategy to adjust heart metabolism and protect the myocardium from impaired contractility. PMID: 24743151
    4. relationships between plasma apoO levels and high-sensitive C-reactive protein (hs-CRP) levels, as well as other lipid parameters in healthy subjects and patients with established acute coronary syndrome PMID: 22693255
    5. apolipoprotein O is the first chondroitin sulfate chain containing apolipoprotein PMID: 16956892

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  • 亚细胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein. Secreted. Mitochondrion. Golgi apparatus membrane. Endoplasmic reticulum membrane.
  • 蛋白家族：
    Apolipoprotein O/MICOS complex subunit Mic27 family
  • 组织特异性：
    Expressed in all tissues examined. Up-regulated in diabetic heart.
  • 数据库链接：

    HGNC: 28727

    OMIM: 300753

    KEGG: hsa:79135

    STRING: 9606.ENSP00000368528

    UniGene: Hs.495851