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  4. Human Beta-Endorphin,β-EP ELISA Kit

Human Beta-Endorphin,β-EP ELISA Kit

  • 中文名称：
    人β内啡肽(β-EP)酶联免疫试剂盒
  • 货号：
    CSB-E06821h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human β-EP ELISA Kit was designed for the quantitative measurement of Human β-EP protein in serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    beta-Endorphin, beta-EP
  • 缩写：
    β-EP
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %99
    Range %91-110
    1:4Average %97
    Range %92-105
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.537 2.568 2.553 2.443
    20002.010 1.999 2.005 1.895
    10001.600 1.532 1.566 1.456
    5001.032 1.122 1.077 0.967
    2500.567 0.600 0.584 0.474
    1250.391 0.410 0.401 0.291
    62.50.242 0.254 0.248 0.138
    00.109 0.111 0.110 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

我想咨询一下人类β-内啡肽ELISA试剂盒（CSB-E06821h）的问题。在规格说明书和使用手册中，我不理解“灵敏度15.6 pg/mL”和“检测范围为62.5 pg/mL至4,000 pg/mL”的差异。在这个ELISA试剂盒中，15.6 pg/mL是最低可检测水平吗？如果是，为什么最低可检测水平（15.6 pg/mL）低于范围的最低水平（62.5 pg/mL）？

A:
L灵敏度15.6pg/m是该试剂盒的最低检测水平，是通过重复测定20次零标准品OD值及其三倍标准差得出的。低于此参数（15.6pg/mL）则无法检测。在15.6-62.5pg/mL区间范围，样品的测量值具有很大的变异性。62.5pg/mL 表示标准品S1的浓度点，而62.5-4000pg/mL是该试剂盒的检测范围。