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Human CLIP-associating protein 2(CLASP2) ELISA kit

  • 中文名称：
    人细胞连接相关蛋白2(CLASP2)酶联免疫试剂盒
  • 货号：
    CSB-EL005472HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CLASP2 ELISA Kit was designed for the quantitative measurement of Human CLASP2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

  • 别名：
    CLASP2; KIAA0627; CLIP-associating protein 2; Cytoplasmic linker-associated protein 2; Protein Orbit homolog 2; hOrbit2
  • 缩写：
    CLASP2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.86 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CLASP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-96
    1:2Average %104
    Range %100-110
    1:4Average %89
    Range %85-93
    1:8Average %86
    Range %81-90
  • 回收率：
    The recovery of human CLASP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9388-97
    EDTA plasma (n=4)9690-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.731 2.612 2.672 2.501
    7502.207 2.129 2.168 1.997
    3751.495 1.408 1.452 1.281
    187.50.804 0.855 0.830 0.659
    940.547 0.514 0.531 0.360
    470.329 0.338 0.334 0.163
    23.50.236 0.229 0.233 0.062
    00.173 0.168 0.171
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
  • 基因功能参考文献：
    1. CLASP2 is involved in the epithelial-mesenchymal transition and progression of bladder urothelial cancer. Simultaneous urine-based detection of CLASP2 and E-cadherin mRNA can efficiently discriminate patients with or without 2-years progression after transurethral resection of the bladder tumor. PMID: 28166762
    2. These findings reveal a new role for Clasp2 in governing keratinocyte undifferentiated features. PMID: 28069833
    3. Prickle1 localized to the membrane through its farnesyl moiety, and the membrane localization was necessary for Prickle1 to regulate migration, to bind to CLASPs and LL5beta, and to promote microtubule targeting of focal adhesions. PMID: 27378169
    4. this study shows that GSK3-mediated phosphorylation of CLASP2alpha largely abolishes CLASP2alpha-microtubule association in metaphase thus contributing to correct chromosome dynamics PMID: 28232523
    5. PAR3 and aPKC control the organization of the Golgi through CLASP2 phosphorylation. PMID: 25518939
    6. GSK3B-dependent phosphorylation and the level of CLASP2 play a role in the maintenance of acetylcholine receptor cluster size through the regulated capture and release of microtubule plus-ends. PMID: 25231989
    7. Propose that CLASPs couple microtubule organization, vesicle transport and cell interactions with the ECM, establishing a local secretion pathway that facilitates focal adhesion turnover by severing cell-matrix connections. PMID: 24859005
    8. Results suggest a previously unidentified role for the scaffolding protein 4.1R in locally controlling CLASP2 behavior, CLASP2 cortical platform turnover and GSK3 activity, enabling correct MT organization and dynamics essential for cell polarity. PMID: 23943871
    9. The epiblast epithelial status was maintained by anchoring microtubules to the basal cortex via CLIP2, a microtubule plus-end tracking protein, and Dystroglycan, a transmembrane protein that bridges the cytoskeleton and basement membrane (BM). PMID: 23940118
    10. Overexpression of human CLASP2 in mouse neurons caused the formation of multiple axons, enhanced dendritic branching, & Golgi condensation. These morphogenetic changes led to significant functional alterations in synaptic transmission. PMID: 23035100
    11. propose that Cdk1 and Plk1 mediate a fine CLASP2 "phospho-switch" that temporally regulates kinetochore-microtubule attachment stability PMID: 23045552
    12. Data show that CENP-E-mediated traction forces on misaligned chromosomes are responsible for the irreversible loss of spindle-pole integrity in CLASP1/2-depleted cells. PMID: 22307330
    13. Regulation of microtubule dynamics by TOG-domain proteins XMAP215/Dis1 and CLASP PMID: 21782439
    14. Data report that NEK1 and CLASP2 colocalize with FEZ1 in a perinuclear region in mammalian cells, and observed that coiled-coil interactions occur between FEZ1/CLASP2 and FEZ1/NEK1 in vitro. PMID: 19924516
    15. propose that CLASP1 and CLASP2 can mediate interactions between microtubule plus ends and the cell cortex and act as local rescue factors, possibly through forming a complex with EB1 at microtubule tips PMID: 15631994
    16. These results demonstrate the striking difference of the microtubule cytoskeleton in the lamella as compared with the cell body and provide the first direct observation of subcellular regulation of a microtubule-associated protein in migrating cells. PMID: 15955847
    17. CLASPs provide apparent stabilization of microtubules by locally reducing the amplitude of growth/shortening episodes at the microtubule ends. PMID: 16866869
    18. Together, our data support that the partial redundancy of CLASPs during mitosis acts as a possible mechanism to prevent aneuploidy in mammals. PMID: 16914514
    19. GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. PMID: 19289791
    20. A role for microtubules that form at the Golgi membranes is identified in a manner dependent on the microtubule regulators CLASPs. PMID: 19701196

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Golgi apparatus, trans-Golgi network. Cell membrane. Cell projection, ruffle membrane.
  • 蛋白家族：
    CLASP family
  • 组织特异性：
    Brain-specific.
  • 数据库链接：

    HGNC: 17078

    OMIM: 605853

    KEGG: hsa:23122

    STRING: 9606.ENSP00000419974

    UniGene: Hs.108614