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Human Calbindin(CALB1) ELISA kit

  • 中文名称:
    人钙结合蛋白(CALB1)酶联免疫试剂盒
  • 货号:
    CSB-EL004432HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human CALB1 ELISA Kit was designed for the quantitative measurement of Human CALB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名:
    avian-type ELISA Kit; CAB27 ELISA Kit; CALB 1 ELISA Kit; CALB ELISA Kit; CALB1 ELISA Kit; CALB1_HUMAN ELISA Kit; Calbindin 1 28kDa ELISA Kit; Calbindin ELISA Kit; Calbindin D28 ELISA Kit; D 28K ELISA Kit; D-28K ELISA Kit; D28K ELISA Kit; OTTHUMP00000166027 ELISA Kit; OTTHUMP00000225441 ELISA Kit; RTVL H protein ELISA Kit; Vitamin D dependent calcium binding protein ELISA Kit; Vitamin D dependent calcium binding protein avian type ELISA Kit; Vitamin D-dependent calcium-binding protein ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human calbindin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %90-99
    1:2Average %92
    Range %88-96
    1:4Average %90
    Range %85-94
    1:8Average %84
    Range %80-88
  • 回收率:
    The recovery of human calbindin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10295-110
    EDTA plasma (n=4)9893-103
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.178 2.302 2.240 2.039
    5001.574 1.532 1.553 1.352
    2501.054 1.076 1.065 0.864
    1250.764 0.791 0.778 0.577
    62.50.478 0.452 0.465 0.264
    31.250.351 0.382 0.367 0.166
    15.60.266 0.258 0.262 0.061
    00.197 0.204 0.201
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
  • 基因功能参考文献:
    1. CSF calbindin concentration is a sensitive biomarker of NPC1 disease PMID: 27307499
    2. We identified a significant association between the A allele of rs1805874 and the risk of Parkinson's disease (OR 1.257, 95% CI 1.036-1.524, p = 0.020). Our findings suggest that the A allele of rs1805874 is associated with risk of Parkinson's disease among the Han Chinese. Our results, combined with previous studies, suggest that rs1805874 is associated with Parkinson's disease in East Asians, but not Caucasians. PMID: 27611799
    3. Age-related loss of CB appears to coincide with tangle formation in the BFCN and is associated with the full range of tau pathology, including late appearing epitopes. PMID: 26417681
    4. Increased number of calbindin neurons and fiber labeling both in focal cortical dysplasia (FCD) and non-FCD specimens compared with postmortem controls may be associated with ongoing seizure activity. PMID: 26081613
    5. expressed by different neuron types, both inhibitory (GABAergic) and excitatory (glutamatergic), involved in both intrinsic and extrinsic circuits of the human cerebellar cortex PMID: 24719368
    6. The calbindin-D 28k was decreased in the putamen, the dorsal tier of the substantia nigra along the lower border of the red nucleus, and in the cerebellar cortex in multiple system atrophy. PMID: 23715974
    7. Significantly reduced hippocampal calbindin D28K levels are found in all patients with mitochondrial encephalomyopathy, lactic acidosis and stroke-like syndrome (MELAS). PMID: 22483853
    8. Data show calbindin (CB)- and tyrosine hydroxylase (TH)-cells were distributed in the three striatal territories, and the density of calretinin (CR) and parvalbumin (PV) interneurons were more abundant in the associative and sensorimotor striatum. PMID: 22272358
    9. Dynamics of Ca2+ ions within the solvation shell of calbindin D9k. PMID: 21364983
    10. upregulated in endometriosis tissues PMID: 20452033
    11. cultured syncytiotrophoblast cells express calbindin-D9k and calbindin-D28k genes, which are stimulated by calcitriol PMID: 20214988
    12. Our study shows there is no association between rs1805874 and risk for Parkinson's disease (PD) in four Caucasian populations. This suggests the effect of calbindin on PD risk displays population specificity. PMID: 19674066
    13. calbindin may play a role in development of Purkinje cells located in heterotopias and cerebellar dysgenesias PMID: 20054781
    14. S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain. PMID: 11994015
    15. binds to myo-inositol-1(or 4)-monophosphatase (IMPase) and increases the phosphatase activity PMID: 12176979
    16. calbindin 1, 28kDa may play a role in Ca2+ transport or cell development in human trophoblast possibly trough Ca2+ buffering PMID: 12606474
    17. The biological function of calbindin D28k appears to be tied to the redox state of its five cysteine residues. PMID: 15641794
    18. Post-translational modifications of human calbindin. PMID: 15741335
    19. Our findings indicate that the expression of parvalbumin or calbindin-D(28k) by subpopulations of dysplastic neurons in cortical tubers is aberrant and denotes dysfunctional inhibitory circuits inept for excitoprotection. PMID: 17156698
    20. aggregates of mutant ataxin-l may recruit calbindin-D28k via tissue transglutaminase 2 mediated covalent crosslinking PMID: 17442486
    21. recombinant human calbindin-D28k was crystallized at 291 K using PEG 3350 as precipitant and a 2.4 A resolution X-ray data set was collected from a single flash-cooled crystal PMID: 18259068
    22. calbindin D(28k) binds Zn2+ to 3 rather strong sites with dissociation constants in low micromolar range. Zn2+-bound state is structurally distinct from Ca2+-bound state, with negative allosteric interaction between Zn- & Ca-binding events. PMID: 18359862
    23. CALB1 is associated with Parkinson's disease independently of alpha-synuclein. PMID: 18568448
    24. No significant differences in cerebellar Purkinje cells in levels of CALB in autism or in the normal cerebellum. PMID: 18587625

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  • 蛋白家族:
    Calbindin family
  • 数据库链接:

    HGNC: 1434

    OMIM: 114050

    KEGG: hsa:793

    STRING: 9606.ENSP00000265431

    UniGene: Hs.65425