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Human Calpain-5(CAPN5) ELISA kit

  • 中文名称：
    人钙蛋白酶5(CAPN5)酶联免疫试剂盒
  • 货号：
    CSB-EL004498HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CAPN5 ELISA Kit was designed for the quantitative measurement of Human CAPN5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    ADNIV ELISA Kit; Calpain htra 3 ELISA Kit; Calpain htra-3 ELISA Kit; Calpain-5 ELISA Kit; CAN5_HUMAN ELISA Kit; CAPN 5 ELISA Kit; Capn5 ELISA Kit; nCL 3 ELISA Kit; nCL-3 ELISA Kit; New calpain 3 ELISA Kit; VRNI ELISA Kit
  • 缩写：
    CAPN5
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CAPN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %81-91
    1:2Average %105
    Range %99-108
    1:4Average %89
    Range %85-93
    1:8Average %93
    Range %89-97
  • 回收率：
    The recovery of human CAPN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9184-95
    EDTA plasma (n=4)9589-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20001.972 1.917 1.945 1.787
    10001.339 1.358 1.349 1.191
    5000.979 0.956 0.968 0.810
    2500.728 0.712 0.720 0.562
    1250.448 0.454 0.451 0.293
    62.50.381 0.369 0.375 0.217
    31.250.261 0.277 0.269 0.111
    00.160 0.156 0.158
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Calcium-regulated non-lysosomal thiol-protease.
  • 基因功能参考文献：
    1. The relative domain rotation of 60-100 degrees we found for mini-calpain-5 (a non-classical calpain) is significantly greater than the largest rotation previously observed for a classical calpain PMID: 27474374
    2. CAPN5 localization at the photoreceptor synapse and with mitochondria explains the neural circuitry phenotype in human CAPN5 disease alleles. PMID: 27152965
    3. A novel CAPN5 (c.750G>T, p.Lys250Asn) missense mutation causes uveitis and neovascular retinal detachment. PMID: 25856303
    4. CAPN5 mutation in hereditary uveitis: the R243L mutation increases calpain catalytic activity and triggers intraocular inflammation PMID: 25994508
    5. CAPN5 expression can be suppressed by shRNA-based RNA interference PMID: 25216694
    6. autosomal dominant neovascular inflammatory vitreoretinopathy is due to CAPN5 gain-of-function rather than haploinsufficiency. PMID: 24381307
    7. Calpain-5 mutations cause autoimmune uveitis, retinal neovascularization, and photoreceptor degeneration. PMID: 23055945
    8. Results describe the cloning and characterization of rat calpain-5, which is highly homologous to human and mouse sequences. PMID: 15464980
    9. CAPN5 polymorphisms are associated with a risk of polycystic ovary syndrome. PMID: 16396936
    10. CAPN5 seems to influence traits related to increased risk for cardiovascular diseases and play a role as a candidate gene for metabolic syndrome PMID: 17227582
    11. We have found significant interaction between CAPN5 and PPARD genes that reduces risk for obesity in 55%. CAPN5 and PPARD gene products may also interact in vivo. PMID: 18657264
    12. Data examine possible allelic imbalance in papillary thyroid cancer at EMSY, CAPN5, and PAK1, as candidate genes within 11q13.5-q14 region using a single nucleotide polymorphism-based analysis. PMID: 18787380
    13. Calpain5 was expressed in endometrial stromal and glandular cells throughout the menstrual cycle and in decidua, and its expression was decreased in both stromal and glandular cells from women with endometriosis compared with that of fertile controls. PMID: 18829447

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  • 相关疾病：
    Vitreoretinopathy, neovascular inflammatory (VRNI)
  • 蛋白家族：
    Peptidase C2 family
  • 组织特异性：
    Expressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer.
  • 数据库链接：

    HGNC: 1482

    OMIM: 193235

    KEGG: hsa:726

    STRING: 9606.ENSP00000278559

    UniGene: Hs.248153