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Human Calponin-2(CNN2) ELISA kit

  • 中文名称：
    人钙调蛋白2(CNN2)酶联免疫试剂盒
  • 货号：
    CSB-EL005656HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CNN2 ELISA Kit was designed for the quantitative measurement of Human CNN2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    Calponin 2 ELISA Kit; Calponin H2 ELISA Kit; Calponin H2, smooth muscle ELISA Kit; Calponin-2 ELISA Kit; Cnn2 ELISA Kit; CNN2_HUMAN ELISA Kit; Neutral calponin ELISA Kit; smooth muscle ELISA Kit
  • 缩写：
    CNN2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CNN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 98  
    Range % 93-103  
    1:4 Average % 95  
    Range % 89-98  
    1:8 Average % 97  
    Range % 92-101  
  • 回收率：
    The recovery of human CNN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 102 98-106  
    EDTA plasma (n=4) 94 86-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.325 2.413 2.369 2.254  
    1000 1.876 1.941 1.909 1.794  
    500 1.374 1.282 1.328 1.213  
    250 0.752 0.764 0.758 0.643  
    125 0.434 0.441 0.438 0.323  
    62.5 0.282 0.297 0.290 0.175  
    31.25 0.183 0.187 0.185 0.070  
    0 0.114 0.115 0.115    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • 基因功能参考文献：
    1. Study in human hepatocellular carcinoma cell line and xenograft mouse model shows that CNN2 plays an important role in tumor growth and metastasis, possibly through MEK1/2-ERK1/2 signaling pathway. PMID: 29333089
    2. our results demonstrated that knockdown of endogenous CNN2 in AGS cells could significantly activate cell apoptosis pathway and therefore suppress cell growth in vitro. The deletion of CNN2 might be a potential therapeutic approach to inhibit aggressive growth of gastric cancer. PMID: 28714360
    3. High Calponin-h2 serum levels are associated with breast cancer. PMID: 25099617
    4. h2-Calponin gene expression is further affected by the treatment of cells with Notch inhibitor and activator, suggesting an upstream signaling mechanism. PMID: 24285540
  • 蛋白家族：
    Calponin family
  • 组织特异性：
    Heart and smooth muscle.
  • 数据库链接：

    HGNC: 2156

    OMIM: 602373

    KEGG: hsa:1265

    STRING: 9606.ENSP00000263097

    UniGene: Hs.651512