Human Collagen Type Ⅳ(COL Ⅳ) ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Human Col Ⅳ ELISA Kit was designed for the quantitative measurement of Human Col Ⅳ protein in serum, plasma, cell culture supernates, urine. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .
别名：

Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
缩写：

Col Ⅳ
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, urine
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	86
	Range %	80-104
1:400	Average %	97
	Range %	91-105
1:800	Average %	100
	Range %	92-113
1:1600	Average %	93
	Range %	86-98

回收率：

The recovery of human COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-102
EDTA plasma (n=4)	93	85-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.