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  4. Human Collagen alpha-2(IV) chain (COL4A2) ELISA kit

Human Collagen alpha-2(IV) chain (COL4A2) ELISA kit

  • 中文名称：
    人IV型胶原α-2链(COL4A2)酶联免疫试剂盒
  • 货号：
    CSB-EL005742HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    Canstatin ELISA Kit; CO4A2_HUMAN ELISA Kit; COL4A 2 ELISA Kit; Col4a2 ELISA Kit; Collagen alpha 2(IV) chain ELISA Kit; Collagen; type IV; alpha 2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    47 pg/mL-3000 pg/mL
  • 灵敏度：
    11.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human COL4A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 92
    Range % 84-95
    1:40 Average % 103
    Range % 96-106
    1:80 Average % 89
    Range % 84-92
    1:160 Average % 106
    Range % 94-109
  • 回收率：
    The recovery of human COL4A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 93-104
    EDTA plasma (n=4) 92 85-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3000 2.661 2.761 2.711 2.611
    1500 2.168 2.243 2.206 2.106
    750 1.774 1.735 1.755 1.655
    375 1.289 1.247 1.268 1.168
    187.5 0.641 0.665 0.653 0.553
    94 0.403 0.411 0.407 0.307
    47 0.240 0.246 0.243 0.143
    0 0.098 0.102 0.100 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
  • 基因功能参考文献：
    1. Herein, we describe a large family of first-degree relatives affected by a novel heterozygous variant in COL4A2 (c.3490G.A). PMID: 28542708
    2. Data showed that Col4A2 can restrain triple-negative breast cancer (TNBC) cell lines MDA-MB-231 and MDA-MB-468 proliferation, migration, cell cycle, and ultimately lead to apoptosis. The data indicated that Col4A2 was significantly correlated with the proliferation and invasive potential of TNBC. PMID: 27906681
    3. In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. PMID: 27919040
    4. These results of this study provide evidence that the mutation of COL4A2 is associated with lacunar ischemic stroke and deep intracerebral hemorrhage. PMID: 28954878
    5. COL4A2 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    6. These results indicate that the CHD-related genetic variant at the COL4A2 locus affects COL4A2/COL4A1 expression, smooth muscle cells survival, and atherosclerotic plaque stability, providing a mechanistic explanation for the association between the genetic variant and CHD risk. PMID: 27389912
    7. Expression of miR-26a and miR-29a was significantly down regulated in leukoplakia and cancer tissues but up regulated in lichen planus tissues. Expression of target genes such as, ADAMTS7, ATP1B1, COL4A2, CPEB3, CDK6, DNMT3a and PI3KR1 was significantly down regulated in at least two of three disease types with respect to normal tissues. PMID: 27515006
    8. identified a novel COL4A2 (c.2399G>A, p.G800E, CCDS41907.1) mutation in an autosomal dominant family with porencephaly and ocular abnormalities PMID: 26708157
    9. No significant change in canstatin levels was observed between normotensive and pre-eclampsia patients. PMID: 26343951
    10. Reduction of COL4A2 expression by RNAI-mediated knockdown induces cell death. Finally, elevated Notch3 expression levels correlate with higher COL4A2 expression in human ovarian tumor specimens PMID: 25169943
    11. An association was found between common variation in the COL4A2 gene and sporadic cerebral small vessel disease. PMID: 25653287
    12. Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. PMID: 24503185
    13. dominant COL4A2 mutations are a novel major risk factor for familial cerebrovascular disease, including porencephaly and small-vessel disease with reduced penetrance and variable phenotype, which might also be modified by other contributing factors. PMID: 22333902
    14. this study confirmed that abnormalities of the alpha1alpha1alpha2 heterotrimers of type IV collagen cause porencephaly and stresses the importance of screening for COL4A2 as well as for COL4A1. PMID: 22209246
    15. COL4A2 was overexpressed in the ER-negative breast cancer group. PMID: 20805453
    16. an imbalance of glomerular alpha2(IV) and alpha5(IV) chain expression occurred in IgA nephropathy PMID: 12021518
    17. Data show that the NC1 but not 7S domain is essential for chain association and initiation of triple helix formation in human collagen alpha2(IV) . PMID: 15522229
    18. alpha2(IV)NC1 domain has a role in regulating tumor cell behavior PMID: 15743801
    19. Comparison of alpha5(IV) with alpha2(IV) expression in Alport patients is simple and eliminates technical artifacts. PMID: 17554254
    20. support a crucial role for angiogenesis inhibitors in shifting the fate of radiation-induced HIF-1alpha activity from hypoxia-induced tumor radioresistance to hypoxia-induced tumor apoptosis PMID: 17557121
    21. The co-detection of alpha5 and alpha2 chains of collagen IV in frozen skin biopsies is therefore proposed as a simple technique to diagnose Alport syndrome. PMID: 18706356
    22. E1B-55kD-deleted oncolytic adenovirus armed with canstatin gene yields an enhanced anti-tumor efficacy on pancreatic cancer. PMID: 19481338

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  • 相关疾病：
    Porencephaly 2 (POREN2); Intracerebral hemorrhage (ICH)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix, basement membrane.
  • 蛋白家族：
    Type IV collagen family
  • 数据库链接：

    HGNC: 2203

    OMIM: 120090

    KEGG: hsa:1284

    STRING: 9606.ENSP00000353654

    UniGene: Hs.508716