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  4. Human Complement Fragment 4d,C4d ELISA Kit

Human Complement Fragment 4d,C4d ELISA Kit

  • 中文名称：
    人补体片段4d(C4d)酶联免疫试剂盒
  • 货号：
    CSB-E14192h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human C4d ELISA Kit was designed for the quantitative measurement of Human C4d protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

  • 缩写：
    C4d
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C4d in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 103
    Range % 94-108
    1:200 Average % 98
    Range % 90-104
    1:400 Average % 89
    Range % 82-93
    1:800 Average % 95
    Range % 88-98
  • 回收率：
    The recovery of human C4d spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 113 103-117
    EDTA plasma (n=4) 94 89-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    50 2.815 2.747 2.781 2.678
    25 2.305 2.232 2.269 2.166
    12.5 1.499 1.537 1.518 1.415
    6.25 0.733 0.745 0.739 0.636
    3.12 0.399 0.413 0.406 0.303
    1.56 0.238 0.231 0.235 0.132
    0.78 0.155 0.166 0.161 0.058
    0 0.101 0.105 0.103 　
  • 数据处理：
  • 货期：
    3-5 working days

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