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  4. Human Complement component C8 alpha chain(C8A) ELISA kit

Human Complement component C8 alpha chain(C8A) ELISA kit

  • 中文名称：
    人补体蛋白C8α(C8A)酶联免疫试剂盒
  • 货号：
    CSB-EL004194HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human C8A ELISA Kit was designed for the quantitative measurement of Human C8A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    C8A ELISA Kit; CO8A_HUMAN ELISA Kit; Complement component 8 subunit alpha ELISA Kit; Complement component 8, alpha polypeptide ELISA Kit; Complement component C8 alpha chain ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C8A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 85
    Range % 80-89
    1:2000 Average % 94
    Range % 90-98
    1:4000 Average % 96
    Range % 92-100
    1:8000 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of human C8A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 94-102
    EDTA plasma (n=4) 104 100-109
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    100 2.563 2.472 2.518 2.373
    50 2.272 2.169 2.221 2.076
    25 1.729 1.684 1.707 1.562
    12.5 1.253 1.293 1.273 1.128
    6.25 0.785 0.801 0.793 0.648
    3.12 0.464 0.462 0.463 0.318
    1.56 0.316 0.320 0.318 0.173
    0 0.141 0.148 0.145 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
  • 基因功能参考文献：
    1. C8 alpha and C8 beta have correspondingly similar roles in MAC-mediated lysis of erythrocytes and bacterial killing. C8 gamma is not required for complement-mediated killing of Gram-negative bacteria. PMID: 12413696
    2. Results describe the crystal structure of the human C8 alpha MACPF domain disulfide-linked to C8 gamma (alphaMACPF-gamma) at 2.15 A resolution. PMID: 18440555
    3. The binding specificity between C8 alpha and C8 beta subunits is determined by a cooperative interaction of the N-terminal thrombospondin type 1 module and the membrane attack complex/perforin domain. PMID: 12220191
    4. Both N-terminal modules in C8 alpha have a role in forming the principal binding site for C9, and binding may be dependent on a cooperative interaction between these modules and the C8 alpha membrane attack complex/perforin domain. PMID: 12463754
    5. result suggests that this segment of C8alpha and corresponding segments of the other MAC family members are independently folded domains PMID: 16618117
    6. One can predict that the indel segment of C8alpha assumes a conformation that allows for multiple points of contact with C8gamma. PMID: 16935577
    7. crystal structure of the MACPF domain of complement component C8alpha at 2.5 angstrom resolution was determined and it was shown that it is structurally homologous to the bacterial, pore-forming, cholesterol-dependent cytolysins PMID: 17872444
    8. C8gamma binds an indel peptide of C8alpha sequence and forms a non-covalent complex. PMID: 19048311

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  • 相关疾病：
    Complement component 8 deficiency, 1 (C8D1)
  • 亚细胞定位：
    Secreted. Cell membrane; Multi-pass membrane protein. Note=Secreted as soluble protein. Inserts into the cell membrane of target cells.
  • 蛋白家族：
    Complement C6/C7/C8/C9 family
  • 数据库链接：

    HGNC: 1352

    OMIM: 120950

    KEGG: hsa:731

    STRING: 9606.ENSP00000354458

    UniGene: Hs.93210